采用无透明带小鼠胚胎试验检测人类体外受精中的内毒素

Detection of endotoxin in human in vitro fertilization by the zona-free mouse embryo assay.

作者信息

Montoro L, Subias E, Young P, Baccaro M, Swanson J, Sueldo C

机构信息

Department of Obstetrics and Gynecology, University of California, San Francisco-Fresno.

出版信息

Fertil Steril. 1990 Jul;54(1):109-12. doi: 10.1016/s0015-0282(16)53645-0.

DOI:10.1016/s0015-0282(16)53645-0

PMID:2358077

Abstract

Endotoxin has been found to be a contaminant in human in vitro fertilization (IVF), leading to low pregnancy rates, mostly through embryo fragmentation. We wished to determine if standard quality control assays (one-cell and two-cell mouse embryo culture) were able to detect the presence of endotoxin and how they compared with the zona-free mouse embryo assays. Endotoxin was detected by the zona-free mouse embryo assays but not by the one- or two-cell mouse embryo assays. When excessive embryo fragmentation is noticed in the human IVF system, the use of zona-free mouse embryo assays is recommended.

摘要

内毒素已被发现是人类体外受精（IVF）中的一种污染物，主要通过胚胎碎片化导致妊娠率低下。我们希望确定标准质量控制检测方法（单细胞和双细胞小鼠胚胎培养）是否能够检测到内毒素的存在，以及它们与无透明带小鼠胚胎检测方法相比如何。无透明带小鼠胚胎检测方法能检测到内毒素，而单细胞或双细胞小鼠胚胎检测方法则不能。当在人类IVF系统中发现过度的胚胎碎片化时，建议使用无透明带小鼠胚胎检测方法。