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发育转录谱分析揭示了关于禾本科植物生殖发育的关键见解。

Developmental transcriptional profiling reveals key insights into Triticeae reproductive development.

机构信息

Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.

出版信息

Plant J. 2013 Jun;74(6):971-88. doi: 10.1111/tpj.12206. Epub 2013 May 11.

Abstract

Despite their importance, there remains a paucity of large-scale gene expression-based studies of reproductive development in species belonging to the Triticeae. As a first step to address this deficiency, a gene expression atlas of triticale reproductive development was generated using the 55K Affymetrix GeneChip(®) wheat genome array. The global transcriptional profiles of the anther/pollen, ovary and stigma were analyzed at concurrent developmental stages, and co-expressed as well as preferentially expressed genes were identified. Data analysis revealed both novel and conserved regulatory factors underlying Triticeae floral development and function. This comprehensive resource rests upon detailed gene annotations, and the expression profiles are readily accessible via a web browser.

摘要

尽管它们很重要,但在属于禾本科的物种的生殖发育的大规模基因表达研究仍然很少。作为解决这一不足的第一步,使用 55K Affymetrix GeneChip®小麦基因组阵列生成了黑麦草生殖发育的基因表达图谱。同时在发育阶段分析了花药/花粉、子房和柱头的整体转录谱,并鉴定了共同表达和优先表达的基因。数据分析揭示了禾本科花发育和功能的新的和保守的调控因子。这个全面的资源依赖于详细的基因注释,并且表达谱可以通过网络浏览器轻松访问。

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