Osaka University, Graduate School of Engineering Science, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
J Biomed Opt. 2013 Jun;18(6):61231. doi: 10.1117/1.JBO.18.6.061231.
Optical assessment of skin burns is possible with second-harmonic-generation (SHG) microscopy due to its high sensitivity to thermal denaturation of collagen molecules. In contrast to previous studies that were performed using excised tissue specimens ex vivo, in vivo observation of dermal collagen fibers in living rat burn models with SHG microscopy is demonstrated. Changes in signal vanishing patterns in the SHG images are confirmed to be dependent on the burn degree. Comparison of the SHG images with Masson's trichrome-stained images indicated that the observed patterns were caused by the coexistence of molten and fibrous structures of dermal collagen fibers. Furthermore, a quantitative parameter for burn assessment based on the depth profile of the mean SHG intensity across the entire SHG image is proposed. These results and discussions imply a potential of SHG microscopy as a minimally invasive, highly quantitative tool for skin burn assessment.
由于其对胶原分子热变性的高灵敏度，二次谐波产生（SHG）显微镜可用于皮肤烧伤的光学评估。与之前使用离体组织标本进行的研究不同，本研究利用 SHG 显微镜对活体大鼠烧伤模型中的真皮胶原纤维进行了体内观察。SHG 图像中信号消失模式的变化被证实与烧伤程度有关。将 SHG 图像与 Masson 三色染色图像进行比较表明，观察到的模式是由真皮胶原纤维的熔融和纤维结构共存引起的。此外，还提出了一种基于整个 SHG 图像的平均 SHG 强度深度分布的烧伤评估定量参数。这些结果和讨论表明，SHG 显微镜作为一种微创、高度定量的皮肤烧伤评估工具具有潜在的应用前景。
