Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, Key Laboratory of Organ Transplantation, Zhejiang University, Hangzhou, Zhejiang Province 310003, China.
Obes Surg. 2013 Sep;23(9):1361-9. doi: 10.1007/s11695-013-0954-7.
The role of intestinal glucose absorption in reducing glycemic levels after duodenojejunal bypass (DJB) is unclear. We hypothesized that DJB surgery decreases intestinal electrogenic glucose absorption by sodium glucose co-transporter 1 (SGLT1), leading to decreased glucose absorption and reduced blood glucose. Six groups of C57/BL6 mice were studied (n = 6 each) 2 weeks, 1 month, and 2 months after DJB or sham surgery.
Daily weight and food intake were measured for 1 month and an oral glucose tolerance test was performed 2 months after surgery. Electrogenic glucose absorption was quantified in an Ussing chamber according to the sodium-dependent increase of short-circuit current (Isc). Intestinal morphology was assessed by hematoxylin and eosin staining. Expression of SGLT1 was determined by quantitative PCR and Western blotting.
The DJB mice lost weight compared with the sham group and exhibited significant adaptive changes, with increased villus height, crypt depth, and villus surface area. Oral glucose absorption was significantly reduced in the DJB mice compared with the sham group. Glucose-induced Isc was significantly lower in the DJB mice than in the sham mice 1 and 2 months after surgery, indicating that SGLT1 activity was reduced after DJB. Transcript levels and protein abundance of SGLT1 after DJB were also decreased compared with the sham group.
DJB surgery reduced intestinal glucose absorption by reducing the activity and expression of the glucose transporter SGLT1, which represents a potential therapeutic target for patients with diabetes.
空肠旁路术（DJB）后肠道葡萄糖吸收在降低血糖水平中的作用尚不清楚。我们假设 DJB 手术通过钠-葡萄糖协同转运蛋白 1（SGLT1）降低肠道电活性葡萄糖吸收，导致葡萄糖吸收减少和血糖降低。研究了 6 组 C57/BL6 小鼠（每组 6 只），在 DJB 或假手术后 2 周、1 个月和 2 个月时进行研究。
在手术后 1 个月测量每日体重和食物摄入量，并进行口服葡萄糖耐量试验。根据钠依赖性短电路电流（Isc）增加，在 Ussing 室中定量葡萄糖吸收。通过苏木精和伊红染色评估肠道形态。通过定量 PCR 和 Western blot 确定 SGLT1 的表达。
DJB 小鼠体重较 sham 组减轻，表现出明显的适应性变化，绒毛高度、隐窝深度和绒毛表面积增加。与 sham 组相比，DJB 组小鼠的口服葡萄糖吸收明显减少。与 sham 组相比，DJB 组小鼠术后 1 和 2 个月时葡萄糖诱导的 Isc 明显降低，表明 SGLT1 活性在 DJB 后降低。与 sham 组相比，DJB 组的 SGLT1 转录水平和蛋白丰度也降低。
DJB 手术通过降低葡萄糖转运蛋白 SGLT1 的活性和表达减少肠道葡萄糖吸收，这代表了糖尿病患者的潜在治疗靶点。
