Li Da-Chao, Yang Jia, Wu Peng, Zhu Rui, Wang Bo, Lin Yuan, Xu Ke-Xin
State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin 300072, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2013 Jan;33(1):142-6.
A new borate polymer PAA-ran-PAAPBA that can specifically adsorb glucose was introduced in the glucose measurement based on surface plasmon resonance, and the high-precision specific detection of glucose concentration was realized. Six layers and twelve layers of borate polymer were respectively bound onto the SPR sensors through the layer-by-layer self-assembly binding method, and the effect of different layers of borate polymer on the glucose surface plasmon resonance measurement was studied. The experiment was conducted in the concentration range of 1-10 mg x dL(-1) (interval delta = 1 mg x dL(-1)), 10-100 mg x dL(-1) (interval delta = 10 mg x dL(-1)), and 100-1 000 mg x dL(-1) (interval delta = 100 mg x dL(-1)), experiment data was fitted by quadric curve and the fitting degree of refractive index difference deltaRU and glucose concentration was obtained. Results showed that the 12-layer-polymer sensor was better than the 6-layer-polymer sensor in the first two smaller ranges, and the measuring result was not significantly affected by layers in the third range, indicating that for the small concentrations increasing polymer layer can dramatically improve the measurement.
一种可特异性吸附葡萄糖的新型硼酸盐聚合物PAA-ran-PAAPBA被引入基于表面等离子体共振的葡萄糖测量中，实现了对葡萄糖浓度的高精度特异性检测。通过层层自组装结合方法将六层和十二层硼酸盐聚合物分别结合到SPR传感器上，研究了不同层数的硼酸盐聚合物对葡萄糖表面等离子体共振测量的影响。实验在1-10 mg·dL⁻¹（间隔δ = 1 mg·dL⁻¹）、10-100 mg·dL⁻¹（间隔δ = 10 mg·dL⁻¹）和100-1000 mg·dL⁻¹（间隔δ = 100 mg·dL⁻¹）的浓度范围内进行，实验数据用二次曲线拟合，得到折射率差δRU与葡萄糖浓度的拟合度。结果表明，在前两个较小范围内，十二层聚合物传感器优于六层聚合物传感器，在第三个范围内测量结果受层数影响不显著，表明对于低浓度，增加聚合物层数可显著改善测量效果。
