Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
J Biosci Bioeng. 2013 Sep;116(3):298-301. doi: 10.1016/j.jbiosc.2013.03.006. Epub 2013 Apr 12.
Streptococcus pyogenes sortase A (SpSrtA) was transduced into mammalian cells. Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. Direct transduction of SpSrtA is expected to be a strong tool for more conditional specific protein modification in mammalian cells.
化脓性链球菌天冬酰胺酰基内切酶 A（SpSrtA）被转导到哺乳动物细胞中。然后通过 Western blot 分析证实 SpSrtA 介导的模型蛋白的细胞内环化呈时间和剂量依赖性。SpSrtA 的直接转导有望成为在哺乳动物细胞中更具条件特异性的蛋白质修饰的有力工具。
