Interfaculty Institute of Biochemistry, University of Tuebingen, Hoppe-Seyler-Str. 4, D-72076 Tuebingen, Germany.
Interfaculty Institute of Biochemistry, University of Tuebingen, Hoppe-Seyler-Str. 4, D-72076 Tuebingen, Germany.
Curr Opin Chem Biol. 2014 Oct;22:122-8. doi: 10.1016/j.cbpa.2014.09.020. Epub 2014 Oct 6.
Sortase-mediated ligation (SML) is one of the most commonly used techniques for the site-specific modification of proteins. Here, a brief overview on advantages and limitations of this technology in comparison with other chemoselective protein modification techniques is provided and successful approaches that extend the application range of SML are discussed.
基于转肽酶的连接（SML）是用于蛋白质定点修饰的最常用技术之一。本文简要概述了该技术相对于其他化学选择性蛋白质修饰技术的优势和局限性，并讨论了扩展 SML 应用范围的成功方法。
