Boerma G J, Liem T L, Jacobs-de Haas J A, de Jong-van Staveren F A, Verbree W, Eman I
Department of Clinical Chemistry, University Hospital Dijkzigt, Rotterdam, The Netherlands.
J Clin Chem Clin Biochem. 1990 Apr;28(4):255-8.
We recently evaluated the Reflotron Total Cholesterol dry chemistry assay by examining the calibration and the accuracy of the assay in serum, whole venous EDTA-blood and capillary EDTA blood. We now describe a study on the analysis in heparinized venous and heparinized finger stick blood. Cholesterol assays in venous serum and plasma, and in finger capillary blood and plasma (all with heparin) were compared. Finger capillary blood was obtained in two ways: 1. in heparinized capillaries; 2. in a Becton Dickinson microtainer that could be centrifuged for separation of the plasma. Venous blood was obtained in plain tubes (for serum) and heparinized tubes to obtain venous whole blood and also venous plasma. We did not, on average, find large differences between the concentrations of cholesterol in the various materials. The regression equations for finger capillary whole blood and venous whole blood however show higher slopes than the others. Inaccuracy due to the sampling technique appears to be no greater for finger capillary samples than for venous samples.
我们最近通过检查血清、静脉全血乙二胺四乙酸（EDTA）抗凝样本和毛细血管EDTA血样中该检测方法的校准和准确性，对Reflotron总胆固醇干化学检测法进行了评估。我们现在描述一项关于肝素化静脉血和肝素化指尖血分析的研究。比较了静脉血清和血浆、指尖毛细血管血和血浆（均含肝素）中的胆固醇检测结果。指尖毛细血管血通过两种方式获取：1. 用肝素化毛细管采集；2. 用可离心分离血浆的Becton Dickinson微量采血管采集。静脉血通过普通试管（用于获取血清）和肝素化试管采集，以获取静脉全血和静脉血浆。总体而言，我们未发现不同样本中胆固醇浓度存在较大差异。然而，指尖毛细血管全血和静脉全血的回归方程斜率比其他方程更高。与静脉样本相比，指尖毛细血管样本因采样技术导致的误差似乎并不大。
