Diksic M, Nagahiro S, Sourkes T L
Montreal Neurological Institute and Hospital, Canada.
J Neural Transm Suppl. 1990;29:131-40. doi: 10.1007/978-3-7091-9050-0_13.
A biological model for the measurement of the rate of serotonin synthesis in rat brain with alpha-[14C]methyl-L-tryptophan is described. The rate of serotonin synthesis in several grossly dissected brain structures is reported. The half-life of the precursor pool, estimated from kinetic data, is between 20 and 25 min. The method allows, for the first time, measurement of the brain serotonin synthesis rate without any pharmacological manipulation and does not require separation of metabolites. Autoradiographic data are also presented to demonstrate anatomical resolution of this method. The synthesis rate can be estimated in a large number of discrete structures when autoradiography is applied. Long retention of the tracer in brain is also demonstrated.
描述了一种用α-[¹⁴C]甲基-L-色氨酸测量大鼠脑内5-羟色胺合成速率的生物学模型。报告了几个大体解剖脑结构中5-羟色胺的合成速率。根据动力学数据估算的前体池半衰期在20至25分钟之间。该方法首次能够在无需任何药理操作的情况下测量脑内5-羟色胺合成速率，且无需分离代谢物。还提供了放射自显影数据以证明该方法的解剖分辨率。应用放射自显影时，可在大量离散结构中估算合成速率。同时也证明了示踪剂在脑内的长时间滞留。
