Huntley G W, Jones E G
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
J Neurocytol. 1990 Apr;19(2):200-12. doi: 10.1007/BF01217298.
Immunoreactivity for two calcium binding proteins, 28 kDa calbindin and parvalbumin, was used to label cells morphologically identical to Cajal-Retzius neurons in the developing visual, prefrontal, sensory-motor and temporal cortex of Old World monkeys. At all fetal ages examined (E110-E155), Cajal-Retzius neurons throughout the cortex were immunoreactive for calbindin as well as being acetylcholinesterase positive. Between E130 and E150, the calbindin-immunoreactive Cajal-Retzius cells in the visual cortex, and a few in other cortical areas, also showed parvalbumin immunoreactivity. A reduced population of immunoreactive Cajal-Retzius cells was detected at birth, and none could be visualized by immunocytochemistry or histochemistry at later postnatal ages. Calbindin and parvalbumin immunoreactivity represents a potentially useful marker for this developmentally regulated population of neurons, and the varied expression of the two proteins suggests that Cajal-Retzius neurons may represent a neurochemically heterogeneous cell population.
利用两种钙结合蛋白（28 kDa钙结合蛋白和小白蛋白）的免疫反应性，对旧大陆猴发育中的视觉、前额叶、感觉运动和颞叶皮质中形态上与Cajal-Retzius神经元相同的细胞进行标记。在所有检查的胎儿年龄（E110-E155），整个皮质的Cajal-Retzius神经元对钙结合蛋白呈免疫反应性，并且乙酰胆碱酯酶呈阳性。在E130至E150之间，视觉皮质中钙结合蛋白免疫反应性的Cajal-Retzius细胞以及其他一些皮质区域中的少数细胞也显示出小白蛋白免疫反应性。出生时检测到免疫反应性Cajal-Retzius细胞数量减少，在出生后的后期，通过免疫细胞化学或组织化学无法观察到任何细胞。钙结合蛋白和小白蛋白免疫反应性是这种发育调控的神经元群体的潜在有用标记，这两种蛋白的不同表达表明Cajal-Retzius神经元可能代表神经化学性质不同的细胞群体。
