Bowles A P, Pantazis C G, Wansley W, Allen M B
Department of Surgery, Medical College of Georgia, Augusta 30912.
J Neurooncol. 1990 Apr;8(2):103-12. doi: 10.1007/BF00177832.
This report describes a new fluorescent microcarrier cytostasis assay. Human glioma cell lines and primary cultures were attached to microcarrier tissue culture beads and treated with various chemotherapeutic drugs. After treatment, the cells were labelled with two vital fluorescent dyes in order to measure cellular viability. The uptake of hydroethidine and Hoechst 33342 was evaluated alone and in combination as probes for determining metabolic activity and cellular proliferation. Hydroethidine was found to be superior when compared to trypan blue and tritiated thymidine. The use of the microcarrier technique allows for the direct cellular measurement of fluorescence without the need of extensive extraction procedures. The fluorescent assay is a sensitive, rapid and an effective way to screen for potential antiproliferative compounds.
本报告描述了一种新的荧光微载体细胞生长抑制测定法。将人胶质瘤细胞系和原代培养物附着于微载体组织培养珠上，并用各种化疗药物进行处理。处理后，用两种活性荧光染料对细胞进行标记，以测量细胞活力。单独及联合评估了氢化乙锭和Hoechst 33342的摄取情况，作为测定代谢活性和细胞增殖的探针。与台盼蓝和氚标记胸腺嘧啶核苷相比，发现氢化乙锭更具优势。微载体技术的使用无需大量提取程序即可直接对细胞进行荧光测量。荧光测定法是筛选潜在抗增殖化合物的一种灵敏、快速且有效的方法。
