Ozerkan K, Atalay M A, Yakut T, Doster Y, Yilmaz E, Karkucak M
Department of Obstetrics and Gynecology, Uludag University, Bursa, Turkey.
Eur J Gynaecol Oncol. 2013;34(1):42-7.
To investigate the polymorphism rates and possible roles of glutathione-s-transferase M1, T1, and P1 gene polymorphisms in the predisposition to endometrial cancer in Caucasian women.
Serum samples and medical records were collected from 53 Caucasian women with newly diagnosed endometrial cancer and 67 women of the same race without any known history of cancer. Multiplex polymerase chain reaction (PCR) analysis was used to assess glutathione-s-transferase M1 (GSTM1) and T1 gene polymorphisms. Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method was used in salvage of GSTP1 gene polymorphism.
Frequencies of GSTM1 and GSTT1 null genotypes were not significantly different between the controls and patients with endometrial cancer (56.7% vs 52.8%, p = 0.671; 32.8% vs 26.4%, p = 0.574, respectively). The authors were not able to demonstrate any association between neither GSTP1 genotypes nor allele frequencies and endometrial carcinoma in the population studied (p = 0.310, p = 0.318, respectively). Moreover, no significant association could be demonstrated with GSTM1 and GSTT1 polymorphisms and clinical stages of endometrial cancer. Nevertheless, there was a significant difference between the frequencies of GSTP1 AA and GG genotypes in relation to Stage I disease when compared with advanced stages of endometrial carcinoma (p = 0.03). In addition, no association was found between polymorphisms of GST suspergene family and non-endometrioid type endometrial carcinomas.
These results suggest that GSTT1, GSTM1, and GSTP1 polymorphisms are not associated with endometrial cancer in the Caucasian population.
研究谷胱甘肽 - S - 转移酶M1、T1和P1基因多态性在白种女性子宫内膜癌易感性中的多态性率及可能作用。
收集53例新诊断为子宫内膜癌的白种女性和67例无任何已知癌症病史的同种族女性的血清样本及病历。采用多重聚合酶链反应（PCR）分析评估谷胱甘肽 - S - 转移酶M1（GSTM1）和T1基因多态性。采用聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）方法检测GSTP1基因多态性。
GSTM1和GSTT1缺失基因型频率在对照组与子宫内膜癌患者之间无显著差异（分别为56.7%对52.8%，p = 0.671；32.8%对26.4%，p = 0.574）。在研究人群中，作者未能证明GSTP1基因型及等位基因频率与子宫内膜癌之间存在任何关联（分别为p = 0.310，p = 0.318）。此外，未发现GSTM1和GSTT1多态性与子宫内膜癌临床分期之间存在显著关联。然而，与子宫内膜癌晚期相比，GSTP1 AA和GG基因型频率在I期疾病中存在显著差异（p = 0.03）。此外，未发现GST超基因家族多态性与非子宫内膜样型子宫内膜癌之间存在关联。
这些结果表明，在白种人群中，GSTT1、GSTM1和GSTP1多态性与子宫内膜癌无关。
