Jordan F L, Wynder H J, Booth P L, Thomas W E
Department of Oral Biology, College of Dentistry, Ohio State University, Columbus 43210.
J Neurosci Res. 1990 May;26(1):74-82. doi: 10.1002/jnr.490260109.
The use of labelled latex beads, with acute incubation conditions, for the identification of active macrophages in mixed cell cultures based on their phagocytic activity is described. Fluorescent beads provided the best results and selectively labelled active macrophage cells in cultures of blood monocytes. When this technique was applied to primary cultures of rat cerebral cortex, a specific cell type was significantly labelled. This was a small round cell, previously uncharacterized, which in addition to phagocytic activity indicated by the ingestion of beads also possessed macrophage biochemical markers. Thus, the procedure appears useful for phagocytic macrophage identification in vitro, and should be generally applicable to any tissue culture system. Additionally, this procedure can be rendered compatible with cell viability and, therefore, be utilized for long-term monitoring of macrophages.
本文描述了在急性孵育条件下，使用标记乳胶珠基于吞噬活性鉴定混合细胞培养物中活性巨噬细胞的方法。荧光珠效果最佳，可选择性地标记血液单核细胞培养物中的活性巨噬细胞。当将该技术应用于大鼠大脑皮层原代培养物时，一种特定细胞类型被显著标记。这是一种此前未被鉴定的小圆形细胞，除了通过吞噬珠子显示出吞噬活性外，还具有巨噬细胞生化标志物。因此，该方法似乎对体外吞噬性巨噬细胞的鉴定有用，并且应普遍适用于任何组织培养系统。此外，该方法可与细胞活力兼容，因此可用于巨噬细胞的长期监测。
