Booth P L, Thomas W E
Department of Oral Biology, College of Dentistry, Ohio State University, Columbus 43210.
Cell Tissue Res. 1991 Dec;266(3):541-51. doi: 10.1007/BF00318596.
Two previously identified forms of macrophage were investigated in primary cultures of cerebral cortical cells. Dynamic features were revealed through time-lapse video recording and aspects of macrophage function were assessed. The two cell forms were shown to be different pre-mitotic stages of a single cell type. The cell cycle for these cells involved an initial large, flat, quiescent cell which retracted to yield a slightly rounded form with numerous processes. This latter form lost processes and developed profuse filopodia as it became very rounded just prior to division; both resulting daughter cells then regained the initial large flat appearance. These cells possessed several properties of macrophages, including phagocytosis, nucleoside diphosphatase enzyme, and CR3 receptors. These properties were transient, expressed just before and after mitosis, but subsequently down-regulated in the flat daughter cells. Because of this feature, it was difficult to determine the exact size of this cell population; however, the observed rate of proliferation suggests it may be substantial. It is suggested that these cells correspond to non-microglial macrophages of brain tissue and, because of their significant down-regulation, they may be difficult to detect. This may be important in studies of brain accessory immune cells in tissue culture.
在大脑皮质细胞原代培养物中研究了两种先前已鉴定出的巨噬细胞形式。通过延时视频记录揭示了其动态特征，并评估了巨噬细胞功能的各个方面。结果表明，这两种细胞形式是单一细胞类型的不同有丝分裂前期阶段。这些细胞的细胞周期包括最初的大而扁平的静止细胞，该细胞收缩后产生带有许多突起的稍圆的形态。后一种形态在分裂前变得非常圆时失去突起并形成大量丝状伪足；随后产生的两个子细胞又恢复了最初的大而扁平的外观。这些细胞具有巨噬细胞的几种特性，包括吞噬作用、核苷二磷酸酶和CR3受体。这些特性是短暂的，在有丝分裂前后表达，但随后在扁平的子细胞中下调。由于这一特征，很难确定这一细胞群体的确切大小；然而，观察到的增殖速率表明其数量可能相当可观。有人认为，这些细胞对应于脑组织中的非小胶质细胞巨噬细胞，并且由于它们的显著下调，可能难以检测到。这在组织培养中脑辅助免疫细胞的研究中可能很重要。
