Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Korea.
PLoS One. 2013 Apr 8;8(4):e61231. doi: 10.1371/journal.pone.0061231. Print 2013.
There are histological and functional differences between human deciduous and permanent periodontal ligament (PDL) tissues. The aim of this study was to determine the differences between these two types of tissue at the molecular level by comparing their gene expression patterns. PDL samples were obtained from permanent premolars (n = 38) and anterior deciduous teeth (n = 31) extracted from 40 healthy persons. Comparative cDNA microarray analysis revealed several differences in gene expression between the deciduous and permanent PDL tissues. These findings were verified by qRT-PCR (quantitative reverse-transcription-polymerase chain reaction) analysis, and the areas where genes are expressed were revealed by immunohistochemical staining. The expressions of 21 genes were up-regulated in deciduous relative to PDL tissues, and those of 30 genes were up-regulated in permanent relative to deciduous PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in the formation of the extracellular matrix (LAMC2, LAMB3, and COMP), tissue development (IGF2BP, MAB21L2, and PAX3), and inflammatory or immune reactions leading to tissue degradation (IL1A, CCL21, and CCL18). The up-regulated genes in permanent PDL tissues were related to tissue degradation (IL6 and ADAMTS18), myocontraction (PDE3B, CASQ2, and MYH10), and neurological responses (FOS, NCAM2, SYT1, SLC22A3, DOCK3, LRRTM1, LRRTM3, PRSS12, and ARPP21). The analysis of differential gene expressions between deciduous and permanent PDL tissues aids our understanding of histological and functional differences between them at the molecular level.
人乳牙和恒牙牙周韧带（PDL）组织在组织学和功能上存在差异。本研究旨在通过比较两种组织的基因表达模式，从分子水平上确定它们之间的差异。从 40 名健康人拔除的恒牙前磨牙（n=38）和前乳牙（n=31）中获取 PDL 样本。比较 cDNA 微阵列分析显示，乳牙和恒牙 PDL 组织之间的基因表达存在几种差异。这些发现通过 qRT-PCR（定量逆转录-聚合酶链反应）分析得到验证，通过免疫组织化学染色显示了基因表达的区域。与 PDL 组织相比，21 个基因在乳牙中上调表达，30 个基因在恒牙中上调表达。在乳牙 PDL 组织中上调表达的基因涉及细胞外基质的形成（LAMC2、LAMB3 和 COMP）、组织发育（IGF2BP、MAB21L2 和 PAX3）以及导致组织降解的炎症或免疫反应（IL1A、CCL21 和 CCL18）。在恒牙 PDL 组织中上调表达的基因与组织降解（IL6 和 ADAMTS18）、肌收缩（PDE3B、CASQ2 和 MYH10）和神经反应（FOS、NCAM2、SYT1、SLC22A3、DOCK3、LRRTM1、LRRTM3、PRSS12 和 ARPP21）有关。乳牙和恒牙 PDL 组织之间差异基因表达的分析有助于我们从分子水平理解它们之间在组织学和功能上的差异。
