Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Korea.
Tissue Eng Part A. 2012 Oct;18(19-20):2040-51. doi: 10.1089/ten.TEA.2011.0318. Epub 2012 Jun 25.
In many studies, adult stem cells have been found in human periodontal ligament (PDL), but in most cases they were found in the permanent teeth. The aim of the present study was to characterize stem cells from the PDL of deciduous teeth (dPDLSCs) and compare them with those from the PDL of permanent teeth (pPDLSCs). Stem cell markers were examined by a flow cytometric analysis. The results of in vitro differentiation into adipogenic and osteogenic lineages were analyzed by histochemical staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results of in vivo transplantation were analyzed by histological staining, immunohistochemical staining, and quantitative RT-PCR. There were no significant differences in the proliferation rate, cell cycle distribution, expressions of stem cell markers such as Stro-1 and CD146, or in vitro differentiation. The pPDLSC transplants made more typical cementum/PDL-like tissues and expressed more cementum/PDL-related genes (CP23 and collagen XII) than did the dPDLSC transplants. Together, these results suggest that pPDLSCs are better candidates for use in reconstructing periodontium.
在许多研究中，已经在人牙周韧带（PDL）中发现了成体干细胞，但在大多数情况下，它们存在于恒牙中。本研究的目的是表征乳牙（dPDLSCs）PDL 中的干细胞，并将其与恒牙（pPDLSCs）PDL 中的干细胞进行比较。通过流式细胞术分析检查干细胞标志物。通过组织化学染色和定量逆转录-聚合酶链反应（RT-PCR）分析体外向成脂和成骨谱系分化的结果。通过组织学染色、免疫组织化学染色和定量 RT-PCR 分析体内移植的结果。增殖率、细胞周期分布、Stro-1 和 CD146 等干细胞标志物的表达以及体外分化没有显著差异。pPDLSC 移植形成了更典型的牙骨质/牙周膜样组织，并表达了更多的牙骨质/牙周膜相关基因（CP23 和胶原 XII），比 dPDLSC 移植多。总之，这些结果表明 pPDLSCs 是重建牙周组织的更好候选者。
