Kumar P, Selvi S Senthamil, Govindaraju M
Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli-24, Tamil Nadu, India.
Asian Pac J Trop Biomed. 2012 Dec;2(12):930-5. doi: 10.1016/S2221-1691(13)60002-7.
To investigate the anti-biofilm and anti-bacterial activity of Junceella juncea (J. juncea) against biofilm forming pathogenic strains.
Gorgonians were extracted with methanol and analysed with fourier transform infrared spectroscopy. Biofilm forming pathogens were identified by Congo red agar supplemented with sucrose. A quantitative spectrophotometric method was used to monitor in vitro biofilm reduction by microtitre plate assay. Anti-bacterial activity of methanolic gorgonian extract (MGE) was carried out by disc diffusion method followed by calculating the percentage of increase with crude methanol (CM).
The presence of active functional group was exemplified by FT-IR spectroscopy. Dry, black, crystalline colonies confirm the production of extracellular polymeric substances responsible for biofilm formation in Congo red agar. MGE exhibited potential anti-biofilm activity against all tested bacterial strains. The anti-bacterial activity of methanolic extract was comparably higher in Salmonella typhii followed by Escherichia coli, Vibrio cholerae and Shigella flexneri. The overall percentage of increase was higher by 50.2% to CM.
To conclude, anti-biofilm and anti-bacterial efficacy of J. juncea is impressive over biofilm producing pathogens and are good source for novel anti-bacterial compounds.
研究琼枝柳珊瑚（Junceella juncea）对形成生物膜的致病菌株的抗生物膜和抗菌活性。
用甲醇提取柳珊瑚并通过傅里叶变换红外光谱进行分析。通过添加蔗糖的刚果红琼脂鉴定形成生物膜的病原体。采用定量分光光度法通过微量滴定板试验监测体外生物膜减少情况。采用纸片扩散法测定甲醇提取物（MGE）的抗菌活性，随后计算与粗甲醇（CM）相比的增加百分比。
傅里叶变换红外光谱证实了活性官能团的存在。干燥、黑色、结晶状菌落证实了在刚果红琼脂中产生了负责生物膜形成的细胞外聚合物。MGE对所有测试菌株均表现出潜在的抗生物膜活性。甲醇提取物对伤寒沙门氏菌的抗菌活性相对较高，其次是大肠杆菌、霍乱弧菌和福氏志贺氏菌。与CM相比，总体增加百分比高出50.2%。
总之，琼枝柳珊瑚对产生生物膜的病原体具有令人印象深刻的抗生物膜和抗菌功效，是新型抗菌化合物的良好来源。
