College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, China.
J Sci Food Agric. 2013 Oct;93(13):3334-8. doi: 10.1002/jsfa.6181. Epub 2013 May 29.
Neotame (NEO) is a new artificial sweetener approved as a food additive in many countries. The present method for the determination of NEO in various foodstuffs is high-performance liquid chromatography (HPLC). There are no reports on the determination of NEO in foods by capillary zone electrophoresis (CZE). Therefore a simple and rapid method to determine NEO is desired for quality control.
A CZE method combined with solid phase extraction was developed for the determination of NEO in non-alcoholic beverage. The optimum separation conditions obtained were 20 mmol L(-1) sodium borate buffer, pH 8, 25 kV applied voltage, 5 s hydrodynamic injection at 30 mbar and ultraviolet detection at 191 nm. The calibration curve showed good linearity (R(2) = 1.000) in the range 0.5-100 µg mL(-1) , and the limit of detection was 0.118 µg mL(-1) . The method was successfully applied to the determination of NEO in two kinds of beverage with migration time less than 5 min, relative standard deviation (n = 3) less than 2% and recoveries ranging from 90 to 95%.
The proposed CZE method has the advantages of shorter analysis time and lower cost compared with HPLC, indicating that it may be a good alternative to the HPLC method.
纽甜（NEO）是一种新型人工甜味剂，已在许多国家获得批准作为食品添加剂。目前，用于测定各种食品中 NEO 的方法是高效液相色谱法（HPLC）。尚无关于毛细管区带电泳（CZE）测定食品中 NEO 的报道。因此，需要一种简单、快速的方法来进行质量控制。
建立了一种 CZE 结合固相萃取法测定非酒精饮料中 NEO 的方法。优化的分离条件为：20mmol/L 硼酸钠缓冲液，pH8.0，25kV 外加电压，30mbar 下 5s 水力进样，191nm 紫外检测。校准曲线在 0.5-100μg/mL 范围内具有良好的线性（R²=1.000），检出限为 0.118μg/mL。该方法成功应用于两种饮料中 NEO 的测定，迁移时间小于 5min，相对标准偏差（n=3）小于 2%，回收率在 90%-95%之间。
与 HPLC 相比，该 CZE 法具有分析时间短、成本低的优点，可能是 HPLC 法的良好替代方法。
