Daniels D H, Joe F L, Warner C R, Fazio T
J Assoc Off Anal Chem. 1984 May-Jun;67(3):513-5.
A liquid chromatographic method is described for the determination of aspartame in dry beverage bases and sweetener tablets. The sample was mixed with the mobile phase, the pH was adjusted to within +/- 0.1 pH unit of the mobile phase, and the sample was diluted to volume with the mobile phase. The solution was filtered and a 10 microL aliquot was injected onto a C18 reverse phase column. Aspartame was quantitated with an ultraviolet detector. Recoveries of aspartame ranged from 94 to 111%. The dry beverage bases contained 5-13% aspartame and the sweetener tablets contained 19% aspartame. The presence of aspartame was confirmed by using thin layer chromatography.
描述了一种用于测定干饮料基料和甜味剂片剂中天冬甜素的液相色谱方法。将样品与流动相混合，将pH值调节至流动相的±0.1个pH单位范围内，并用流动相将样品稀释至规定体积。溶液经过过滤，取10微升等分试样注入C18反相柱。使用紫外检测器对天冬甜素进行定量。天冬甜素的回收率在94%至111%之间。干饮料基料中天冬甜素的含量为5%至13%，甜味剂片剂中天冬甜素的含量为19%。通过薄层色谱法确认了天冬甜素的存在。
