Sypecka Joanna, Sarnowska Anna, Gadomska-Szabłowska Ilona, Lukomska Barbara, Domańska-Janik Krystyna
NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
Acta Neurobiol Exp (Wars). 2013;73(1):116-29. doi: 10.55782/ane-2013-1926.
The NG2-positive cells are the oligodendrocyte precursors, which, when terminally differentiated, are capable of myelinating the central nervous system. There is however an ever-growing list of evidences that NG2 cells actually possess an intrinsic neurogenic potential and they are capable of neuronal differentiation in response to environmental stimuli. To address the question, we have established a model of an indirect co-culture system of the freshly isolated rat neonatal NG2 cells and organotypic slices derived from two distinct CNS regions (hippocampus and spinal cord) to mimic the nervous tissue microenviroment. The cell differentiation in microenvironment of OGD-injured hippocampal slices has been studied as well. The molecular analysis of selected trophic factors has been performed to determine the patterns of their expression. Indeed, the comparison of the cell commitment and development in various microenvironments has pointed to significant dissimilarities. First of all, the medium being continuously conditioned by the hippocampal slices efficiently promoted neurogenesis. The effect has been significantly abolished in co-cultures with the injured tissue. The less pronounced susceptibility to adopting neuronal phenotype and the considerable slowdown of oligodendroglial development was observed in the co-cultures with the spinal cord slices. The role of BDNF in oligodendroglial progenitor commitment and development has been investigated proving that it is one of the key players in the examined processes. The specificity of the instructive clues cocktail might module the fate choice of mobilized endogenous or transplanted cells, which should be taken into consideration while planning neurorepair strategies.
NG2 阳性细胞是少突胶质细胞前体，当其终末分化时，能够使中枢神经系统形成髓鞘。然而，越来越多的证据表明，NG2 细胞实际上具有内在的神经发生潜能，并且它们能够响应环境刺激而发生神经元分化。为了解决这个问题，我们建立了一个间接共培养系统模型，该模型将新鲜分离的大鼠新生 NG2 细胞与源自两个不同中枢神经系统区域（海马体和脊髓）的器官型切片进行共培养，以模拟神经组织微环境。我们还研究了氧糖剥夺损伤海马体切片微环境中的细胞分化情况。我们对选定的营养因子进行了分子分析，以确定它们的表达模式。事实上，对各种微环境中细胞定向分化和发育的比较表明存在显著差异。首先，由海马体切片持续调节的培养基有效地促进了神经发生。在与损伤组织的共培养中，这种作用被显著消除。在与脊髓切片的共培养中，观察到对采用神经元表型的敏感性较低，以及少突胶质细胞发育明显减缓。我们研究了脑源性神经营养因子（BDNF）在少突胶质细胞祖细胞定向分化和发育中的作用，证明它是所研究过程中的关键因素之一。指导性线索混合物的特异性可能会调节动员的内源性或移植细胞的命运选择，在规划神经修复策略时应考虑到这一点。
