Pandamooz Sareh, Nabiuni Mohammad, Miyan Jaleel, Ahmadiani Abolhassan, Dargahi Leila
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Mol Neurobiol. 2016 Sep;53(7):4659-74. doi: 10.1007/s12035-015-9403-z. Epub 2015 Aug 27.
Recent improvements in organotypic slice culturing and its accompanying technological innovations have made this biological preparation increasingly useful ex vivo experimental model. Among organotypic slice cultures obtained from various central nervous regions, spinal cord slice culture is an absorbing model that represents several unique advantages over other current in vitro and in vivo models. The culture of developing spinal cord slices, as allows real-time observation of embryonic cells behaviors, is an instrumental platform for developmental investigation. Importantly, due to the ability of ex vivo models to recapitulate different aspects of corresponding in vivo conditions, these models have been subject of various manipulations to derive disease-relevant slice models. Moreover spinal cord slice cultures represent a potential platform for screening of different pharmacological agents and evaluation of cell transplantation and neuroregenerative materials. In this review, we will focus on studies carried out using the ex vivo model of spinal cord slice cultures and main advantages linked to practicality of these slices in both normal and neuropathological diseases and summarize them in different categories based on application.
近年来，器官型切片培养技术及其相关技术创新取得了进展，使这种生物制剂成为越来越有用的体外实验模型。在从不同中枢神经区域获得的器官型切片培养物中，脊髓切片培养是一个引人关注的模型，与目前其他体外和体内模型相比具有几个独特优势。发育中的脊髓切片培养能够实时观察胚胎细胞行为，是发育研究的重要平台。重要的是，由于体外模型能够重现相应体内条件的不同方面，这些模型已被用于各种操作以衍生出与疾病相关的切片模型。此外，脊髓切片培养是筛选不同药理试剂以及评估细胞移植和神经再生材料的潜在平台。在本综述中，我们将重点关注使用脊髓切片培养的体外模型进行的研究，以及这些切片在正常和神经病理学疾病中的实用性所带来的主要优势，并根据应用将它们归纳为不同类别。
