An Guang-zhou, Zhou Yan, Hou Qing-xia, Li Yu-rong, Jiang Da-peng, Guo Guo-zhen, Zhang Chen, Ding Gui-rong
Department of Radiation Medicine, College of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013 Apr;31(4):246-50.
To investigate the effect of long-term power frequency electromagnetic field (50 Hz) exposure on the proliferation and apoptosis of human lens epithelial cells (SRA01/04 cells).
SRA01/04 cells in the exponential growth phase were exposed or sham-exposed to power frequency electromagnetic field (50 Hz, 2.3 mT) for 2 hours per day, 5 days every week. After 11 weeks of exposure, the cells were collected; the cell morphology was observed under a microscope, the cell viability was measured by MTT assay, the cell cycle and apoptosis were examined by flow cytometry, and the protein expression levels of cyclin D and proliferating cell nuclear antigen (PCNA) were determined by western blot.
Compared with the sham-exposed SRA01/04 cells, most exposed cells became rounded and more stereoscopic, and heterochromatin gathered near the nuclear membrane in some exposed cells. The MTT assay showed that the viability of exposed cells was significantly increased compared with that of the sham-exposed cells (P < 0.05). Long-term power frequency electromagnetic field exposure led to significantly increased number of cells in S phase (P < 0.05), and the proliferation index was significantly higher in the exposed cells than in the sham-exposed cells (P < 0.05). There was no significant difference in apoptotic rate between the exposed cells and sham-exposed cells (P > 0.05). The exposed cells had significantly higher protein expression levels of cyclin D and PCNA than the sham-exposed cells (P < 0.05).
Long-term power frequency electromagnetic field exposure can promote cellular proliferation and change cell cycle in SRA01/04 cells, but it has no marked effect on the apoptosis of SRA01/04 cells.
探讨长期暴露于工频电磁场（50Hz）对人晶状体上皮细胞（SRA01/04细胞）增殖和凋亡的影响。
将处于指数生长期的SRA01/04细胞每天暴露或假暴露于工频电磁场（50Hz，2.3mT）2小时，每周5天。暴露11周后收集细胞；在显微镜下观察细胞形态，采用MTT法检测细胞活力，通过流式细胞术检测细胞周期和凋亡情况，并用蛋白质印迹法测定细胞周期蛋白D和增殖细胞核抗原（PCNA）的蛋白表达水平。
与假暴露的SRA01/04细胞相比，大多数暴露细胞变得圆润且立体感更强，部分暴露细胞的异染色质聚集在核膜附近。MTT法检测显示，与假暴露细胞相比，暴露细胞的活力显著增加（P<0.05）。长期工频电磁场暴露导致S期细胞数量显著增加（P<0.05），且暴露细胞的增殖指数显著高于假暴露细胞（P<0.05）。暴露细胞与假暴露细胞的凋亡率无显著差异（P>0.05）。暴露细胞的细胞周期蛋白D和PCNA的蛋白表达水平显著高于假暴露细胞（P<0.05）。
长期工频电磁场暴露可促进SRA01/04细胞的细胞增殖并改变细胞周期，但对SRA01/04细胞的凋亡无明显影响。
