Department of Biomedical Engineering, Dongguk University, Seoul, 100-715, South Korea.
Department of Chemistry, Dongguk University, Seoul, 100-715, South Korea.
Sci Rep. 2019 Feb 4;9(1):1333. doi: 10.1038/s41598-018-37372-2.
It has been reported that hypomagnetic fields (HMFs) have a negative influence on mammalian physiological functions. We previously reported that HMFs were detrimental to cell fate changes during reprogramming into pluripotency. These studies led us to investigate whether HMFs affect cell fate determination during direct differentiation. Here, we found that an HMF environment attenuates differentiation capacity and is detrimental to cell fate changes during the in vitro differentiation of embryonic stem cells (ESCs). Moreover, HMF conditions cause abnormal DNA methylation through the dysregulation of DNA methyltransferase3b (Dnmt3b) expression, eventually resulting in incomplete DNA methylation during differentiation. Taken together, these results suggest that an appropriate electromagnetic field (EMF) environment may be essential for favorable epigenetic remodeling during cell fate determination via differentiation.
据报道，低磁环境 (HMFs) 对哺乳动物的生理功能有负面影响。我们之前的研究表明，HMFs 不利于细胞在重编程为多能性过程中的命运变化。这些研究促使我们研究 HMFs 是否会影响直接分化过程中的细胞命运决定。在这里，我们发现 HMF 环境会减弱分化能力，并在胚胎干细胞 (ESCs) 的体外分化过程中不利于细胞命运的改变。此外，HMF 条件通过失调 DNA 甲基转移酶 3b (Dnmt3b) 的表达引起异常的 DNA 甲基化，最终导致分化过程中不完全的 DNA 甲基化。总之，这些结果表明，在通过分化进行细胞命运决定时，适当的电磁场 (EMF) 环境可能对有利的表观遗传重塑至关重要。
