University Hospital of Bordeaux, Virology Laboratory and Biology Department, Bordeaux, France.
J Med Virol. 2013 Jul;85(7):1222-8. doi: 10.1002/jmv.23573. Epub 2013 Apr 17.
Financial and operational constraints limit low-resource countries in the screening of high-risk genital human papillomaviruses (HR-HPV), the etiological agents of cervical cancer. With its simple storage, conservation and shipping, dried cervical sample (DCS) could represent an efficient tool. The aim of the study was to evaluate the reliability of HPV genotyping from DCS. Cervical samples were obtained from 50 women infected with HIV-1 in Côte d'Ivoire. After DNA extraction from both DCS and matched liquid cervical samples (LCS), HPV genotyping was performed and the concordance of genotyping results was evaluated. HPV prevalence was 88% in LCS and 78% in DCS. Kappa statistic was 0.51 for the presence of any genotype (95% confidence interval, 0.25-0.77) and 0.73 for HR-HPV (0.45-0.99). Out of 50 samples, 45 were HPV-positive for DCS and/or LCS, and HR-HPV were detected in 37 samples (74%) with 36 HR-HPV multiple infections. Any genotype and HR genotype identification was concordant/compatible in 86% (43/50) and 88% (44/50) of samples, respectively. In most instances, kappa statistics for detection of type-specific HPV was over 0.6 (including HPV-16, -18, -31, -33). An excellent agreement (kappa statistic ≥ 0.81) was found for eight genotypes (HPV-6, -31, -35, -40, -56, -58, -66, and -82). In spite of interfering factors (multiple infections, different HPV loads, amplification competition, different inputs), DCS and LCS led to concordant/compatible results in most cases. DCS could represent an efficient tool for epidemiological field studies in resource-limited settings, and more importantly for improving the screening coverage and care management in women infected with HPV.
经济和运营方面的限制使得资源匮乏的国家难以对高危型生殖器人乳头瘤病毒（HR-HPV）进行筛查，而 HR-HPV 正是宫颈癌的致病原。干燥宫颈样本（DCS）因其储存、保存和运输简便，可能成为一种有效的工具。本研究旨在评估 DCS 进行 HPV 基因分型的可靠性。采集科特迪瓦 50 名感染 HIV-1 的女性的宫颈样本。从 DCS 和匹配的宫颈液基样本（LCS）中提取 DNA 后，进行 HPV 基因分型，并评估基因分型结果的一致性。LCS 中 HPV 总感染率为 88%，DCS 中 HPV 总感染率为 78%。任何型别 HPV 存在的 Kappa 统计量为 0.51（95%置信区间，0.25-0.77），高危型 HPV 为 0.73（0.45-0.99）。50 份样本中，45 份 DCS 和/或 LCS 为 HPV 阳性，37 份（74%）样本检测到 HR-HPV，其中 36 份为 HR-HPV 多重感染。86%（43/50）和 88%（44/50）的样本中，DCS 和 LCS 对任何型别和 HR 型别 HPV 的鉴定结果均一致/相符。在大多数情况下，检测特定 HPV 型别的 Kappa 统计量超过 0.6（包括 HPV-16、-18、-31、-33）。8 种 HPV 型别（HPV-6、-31、-35、-40、-56、-58、-66 和 -82）的一致性非常好（Kappa 统计量≥0.81）。尽管存在多种感染、HPV 载量不同、扩增竞争和不同的输入等干扰因素，但在大多数情况下，DCS 和 LCS 得到的结果一致/相符。DCS 可为资源匮乏地区的流行病学研究提供一种有效的工具，更重要的是，可提高 HPV 感染女性的筛查覆盖率和护理管理水平。
