Alfarsi Mohammed A, Hamlet Stephen M, Ivanovski Saso
School of Dentistry and Oral Health, Griffith Health Institute, Griffith University, Gold Coast, Australia; College of Dentistry, King Khalid University, Abha, Saudi Arabia.
J Biomed Mater Res A. 2014 Jan;102(1):60-7. doi: 10.1002/jbm.a.34666. Epub 2013 Apr 18.
Increased titanium surface hydrophilicity has been shown to accelerate dental implant osseointegration. Macrophages are important in the early inflammatory response to surgical implant placement and influence the subsequent healing response. This study investigated the modulatory effect of a hydrophilic titanium surface on the inflammatory cytokine expression profile in a human macrophage cell line (THP-1). Genes for 84 cytokines, chemokines, and their receptors were analyzed following exposure to (1) polished (SMO), (2) micro-rough sand blasted, acid etched (SLA), and (3) hydrophilic-modified SLA (modSLA) titanium surfaces for 1 and 3 days. By day 3, the SLA surface elicited a pro-inflammatory response compared to the SMO surface with statistically significant up-regulation of 16 genes [Tumor necrosis factor (TNF) Interleukin (IL)-1β, Chemokine (C-C motif) ligand (CCL)-1, 2, 3, 4, 18, 19, and 20, Chemokine (C-X-C motif) ligand (CXCL)-1, 5, 8 and 12, Chemokine (C-C motif) receptor (CCR)-7, Lymphotoxin-beta (LTB), and Leukotriene B4 receptor (LTB4R)]. This effect was countered by the modSLA surface, which down-regulated the expression of 10 genes (TNF, IL-1α and β, CCL-1, 3, 19 and 20, CXCL-1 and 8, and IL-1 receptor type 1), while two were up-regulated (osteopontin and CCR5) compared to the SLA surface. These cytokine gene expression changes were confirmed by decreased levels of corresponding protein secretion in response to modSLA compared to SLA. These results show that a hydrophilic titanium surface can modulate human macrophage pro-inflammatory cytokine gene expression and protein secretion. An attenuated pro-inflammatory response may be an important molecular mechanism for faster and/or improved wound healing.
钛表面亲水性增加已被证明可加速牙种植体的骨整合。巨噬细胞在对手术植入种植体的早期炎症反应中起重要作用，并影响随后的愈合反应。本研究调查了亲水性钛表面对人巨噬细胞系（THP-1）中炎性细胞因子表达谱的调节作用。在暴露于（1）抛光（SMO）、（2）微粗糙喷砂、酸蚀（SLA）和（3）亲水性改性SLA（modSLA）钛表面1天和3天后，分析了84种细胞因子、趋化因子及其受体的基因。到第3天，与SMO表面相比，SLA表面引发了促炎反应，16个基因 [肿瘤坏死因子（TNF）、白细胞介素（IL）-1β、趋化因子（C-C基序）配体（CCL）-1、2、3、4、18、19和20、趋化因子（C-X-C基序）配体（CXCL）-1、5、8和12、趋化因子（C-C基序）受体（CCR）-7、淋巴毒素-β（LTB）和白三烯B₄受体（LTB4R）] 有统计学意义的上调。modSLA表面抵消了这种作用，与SLA表面相比，它下调了10个基因（TNF、IL-1α和β、CCL-1、3、19和20、CXCL-1和8以及IL-1受体1型）的表达，而有两个基因（骨桥蛋白和CCR5）上调。与SLA相比，这些细胞因子基因表达的变化通过对modSLA反应时相应蛋白质分泌水平的降低得到证实。这些结果表明，亲水性钛表面可调节人巨噬细胞促炎细胞因子基因表达和蛋白质分泌。促炎反应减弱可能是伤口愈合更快和/或更好的重要分子机制。
