School of Dentistry and Oral Health, Centre for Medicine and Oral Health, Griffith University, Gold Coast Campus, Gold Coast, QLD, Australia.
Clin Oral Implants Res. 2012 May;23(5):584-90. doi: 10.1111/j.1600-0501.2011.02325.x. Epub 2011 Nov 1.
Chemical modification of microrough titanium dental implants to produce a hydrophilic surface with increased wettability and improved surface energy has been demonstrated clinically to achieve superior bone wound healing and osseointegration compared to that achieved with a microrough titanium surface alone. As the recruitment of the necessary osseoinductive precursors involved in bone wound healing and osseointegration to the wound site is facilitated by the action of cytokines, this study sought to determine the in vitro effect of hydrophilic surface modification on the expression of pro-inflammatory cytokines from adherent macrophages.
The surface topography and composition of the titanium surfaces was characterized by scanning electron microscopy and X-ray photoelectron spectroscopy. Macrophage attachment and proliferation was assessed using an MTT assay. The expression of 84 pro-inflammatory cytokines and chemokines by adherent RAW 264.7 cells, a murine leukaemic monocyte cell line, was assessed by PCR array after 24 h culture on either smooth polished, sand-blasted acid-etched (SLA) or hydrophilic-modified SLA (SLActive) titanium surfaces.
Following 24 h culture on titanium, surface microroughness activated pro-inflammatory cytokine gene transcription in RAW 264.7 cells. Although there was no significant difference in the degree of cellular attachment or proliferation of RAW 264.7 cells to the different titanium surfaces, by 24 h the hydrophilic surface elicited a gene expression profile with significant down-regulation of the key pro-inflammatory cytokines Tnfα, IL-1α, IL-1β and the chemokine Ccl-2.
Down-regulation of the expression of pro-inflammatory cytokine genes may thus modulate the inflammatory response and may facilitate the enhanced bone wound healing and osseointegration observed clinically using implants with a microrough hydrophilic surface.
化学修饰微粗糙钛牙科植入物以产生亲水性表面，提高润湿性和表面能，已在临床上证明可实现优于单独使用微粗糙钛表面的骨创伤愈合和骨整合。由于细胞因子的作用促进了与骨创伤愈合和骨整合相关的必需的骨诱导前体向创伤部位的募集，因此本研究旨在确定亲水表面修饰对附着巨噬细胞中促炎细胞因子表达的体外影响。
通过扫描电子显微镜和 X 射线光电子能谱对钛表面的表面形貌和组成进行了表征。通过 MTT 测定法评估了巨噬细胞的附着和增殖。在光滑抛光、喷砂酸蚀（SLA）或亲水改性 SLA（SLActive）钛表面上培养 24 小时后，通过 PCR 阵列评估附着的 RAW 264.7 细胞（一种鼠白血病单核细胞系）中 84 种促炎细胞因子和趋化因子的表达。
在钛上培养 24 小时后，表面微粗糙度激活了 RAW 264.7 细胞中促炎细胞因子基因的转录。尽管 RAW 264.7 细胞在不同钛表面的附着或增殖程度没有显著差异，但在 24 小时时，亲水表面引发了基因表达谱，关键的促炎细胞因子 Tnfα、IL-1α、IL-1β 和趋化因子 Ccl-2 的表达显著下调。
因此，促炎细胞因子基因表达的下调可能调节炎症反应，并促进临床上使用微粗糙亲水表面的植入物观察到的增强的骨创伤愈合和骨整合。
