Zhang Yufeng, Li Shue, Wu Chengtie
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, People's Republic of China; Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, People's Republic of China.
J Biomed Mater Res A. 2014 Jan;102(1):105-16. doi: 10.1002/jbm.a.34679. Epub 2013 Apr 18.
The goal of periodontal tissue engineering is to regenerate alveolar bone, root cementum and periodontal ligament. To achieve this goal, bioactive scaffolds play an important role in inducing in vitro osteogenic/cementogenic gene expression of periodontal ligament cells (PDLCs) and in vivo bone/cementum formation. Diopside (DIOP: CaMgSi2O6) ceramics have shown excellent in vitro bioactivity for potential bone repair application. However, there is no study about DIOP porous scaffolds for periodontal tissue engineering. The aim of this study is to prepare DIOP scaffolds and investigate their in vitro and in vivo osteogenesis/cementogenesis for periodontal regeneration application. DIOP scaffolds with highly porous architecture were prepared and β-tricalcium phosphate (β-TCP) scaffolds were used for the control. The interaction of DIOP scaffolds with PDLCs was studied by investigating cell attachment, proliferation and ostegenic/cementogenic differentiation of PDLCs. DIOP scaffolds were implanted into the periodontal defects of beagle dogs to evaluate their in vivo osteogenesis/cementogenesis by hematoxylin and eosin (H&E), tartrate-resistant acid phosphatase staining, and immunohistochemistry (type I collagen: Col I; cementum attachment protein) analyses. The results have shown that DIOP scaffolds supported the attachment and proliferation of PDLCs. DIOP scaffolds significantly enhanced osteogenesis/cementogenesis-related gene expression (Col 1, Runx2, transforming growth factor beta 1, and bone morphogenetic protein 2) of PDLCs, compared to β-TCP scaffolds. The in vivo study showed that DIOP scaffolds induced new bone and cementum regeneration of periodontal tissue defects. The rate of new bone and cementum in DIOP scaffolds is comparable to that in conventional β-TCP scaffolds. Our results indicated that silicate-based DIOP ceramics could not only be used for bone tissue engineering, but also for periodontal tissue engineering due to their excellent in vitro and in vivo osteogeneis/cementogenesis.
牙周组织工程的目标是再生牙槽骨、牙根骨质和牙周韧带。为实现这一目标，生物活性支架在诱导牙周膜细胞（PDLCs）的体外成骨/成牙骨质基因表达以及体内骨/牙骨质形成方面发挥着重要作用。透辉石（DIOP：CaMgSi2O6）陶瓷已显示出在潜在骨修复应用方面优异的体外生物活性。然而，尚无关于用于牙周组织工程的DIOP多孔支架的研究。本研究的目的是制备DIOP支架，并研究其在体外和体内用于牙周再生的成骨/成牙骨质能力。制备了具有高度多孔结构的DIOP支架，并使用β - 磷酸三钙（β-TCP）支架作为对照。通过研究PDLCs的细胞附着、增殖和成骨/成牙骨质分化，来探讨DIOP支架与PDLCs的相互作用。将DIOP支架植入比格犬的牙周缺损处，通过苏木精和伊红（H&E）染色、抗酒石酸酸性磷酸酶染色以及免疫组织化学（I型胶原：Col I；牙骨质附着蛋白）分析来评估其体内成骨/成牙骨质能力。结果表明，DIOP支架支持PDLCs的附着和增殖。与β-TCP支架相比，DIOP支架显著增强了PDLCs的成骨/成牙骨质相关基因表达（Col 1、Runx2、转化生长因子β1和骨形态发生蛋白2）。体内研究表明，DIOP支架可诱导牙周组织缺损处新骨和牙骨质再生。DIOP支架中的新骨和牙骨质生成率与传统β-TCP支架相当。我们的结果表明，基于硅酸盐的DIOP陶瓷不仅可用于骨组织工程，还因其优异的体外和体内成骨/成牙骨质能力而可用于牙周组织工程。
