牛磺胆酸钠共转运蛋白介导绒猴乙型肝炎病毒感染食蟹猴肝细胞。
Sodium taurocholate cotransporting polypeptide mediates woolly monkey hepatitis B virus infection of Tupaia hepatocytes.
机构信息
National Institute of Biological Sciences, Beijing, China.
出版信息
J Virol. 2013 Jun;87(12):7176-84. doi: 10.1128/JVI.03533-12. Epub 2013 Apr 17.
Abstract
Primary Tupaia hepatocytes (PTHs) are susceptible to woolly monkey hepatitis B virus (WMHBV) infection, but the identity of the cellular receptor(s) mediating WMHBV infection of PTHs remains unclear. Recently, sodium taurocholate cotransporting polypeptide (NTCP) was identified as a functional receptor for human hepatitis B virus (HBV) infection of primary human and Tupaia hepatocytes. In this study, a synthetic pre-S1 peptide from WMHBV was found to bind specifically to cells expressing Tupaia NTCP (tsNTCP) and it efficiently blocked WMHBV entry into PTHs; silencing of tsNTCP in PTHs significantly inhibited WMHBV infection. Ectopic expression of tsNTCP rendered HepG2 cells susceptible to WMHBV infection. These data demonstrate that tsNTCP is a functional receptor for WMHBV infection of PTHs. The result also indicates that NTCP's orthologs likely act as a common cellular receptor for all known primate hepadnaviruses.
摘要
原代食蟹猴肝细胞（PTHs）易感染绒猴乙型肝炎病毒（WMHBV），但介导 WMHBV 感染 PTHs 的细胞受体（s）的身份仍不清楚。最近，牛磺胆酸钠共转运蛋白（NTCP）被鉴定为人类乙型肝炎病毒（HBV）感染原代人和食蟹猴肝细胞的功能受体。在这项研究中，发现 WMHBV 的合成前 S1 肽特异性结合表达食蟹猴 NTCP（tsNTCP）的细胞，并且有效地阻止 WMHBV 进入 PTHs；在 PTHs 中沉默 tsNTCP 可显著抑制 WMHBV 感染。tsNTCP 的异位表达使 HepG2 细胞易感染 WMHBV。这些数据表明 tsNTCP 是 PTHs 感染 WMHBV 的功能受体。该结果还表明，NTCP 的同源物可能作为所有已知灵长类动物嗜肝病毒的共同细胞受体发挥作用。
相似文献
1
Sodium taurocholate cotransporting polypeptide mediates woolly monkey hepatitis B virus infection of Tupaia hepatocytes.牛磺胆酸钠共转运蛋白介导绒猴乙型肝炎病毒感染食蟹猴肝细胞。
J Virol. 2013 Jun;87(12):7176-84. doi: 10.1128/JVI.03533-12. Epub 2013 Apr 17.
2
Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus.牛磺胆酸钠共转运多肽是乙型肝炎病毒和丁型肝炎病毒的功能性受体。
Elife. 2012 Nov 13;1:e00049. doi: 10.7554/eLife.00049.
3
Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus.牛磺胆酸钠共转运多肽是人类乙型和丁型肝炎病毒的功能性受体。
Elife. 2012 Nov 13;3. doi: 10.7554/eLife.00049.
4
Binding of Hepatitis B Virus Pre-S1 Domain-Derived Synthetic Myristoylated Peptide to Scavenger Receptor Class B Type 1 with Differential Properties from Sodium Taurocholate Cotransporting Polypeptide.乙型肝炎病毒前 S1 结构域衍生的合成豆蔻酰化肽与清道夫受体 B 类 1 结合的特性不同于牛磺胆酸钠共转运多肽。
Viruses. 2022 Jan 7;14(1):105. doi: 10.3390/v14010105.
5
Unusual Features of Sodium Taurocholate Cotransporting Polypeptide as a Hepatitis B Virus Receptor.牛磺胆酸钠共转运多肽作为乙型肝炎病毒受体的独特特征
J Virol. 2016 Aug 26;90(18):8302-13. doi: 10.1128/JVI.01153-16. Print 2016 Sep 15.
6
Kinetics of the bile acid transporter and hepatitis B virus receptor Na+/taurocholate cotransporting polypeptide (NTCP) in hepatocytes.肝细胞中胆汁酸转运蛋白和乙型肝炎病毒受体钠离子/牛磺胆酸钠共转运多肽（NTCP）的动力学。
J Hepatol. 2014 Oct;61(4):867-75. doi: 10.1016/j.jhep.2014.05.018. Epub 2014 May 15.
7
Evaluation and identification of hepatitis B virus entry inhibitors using HepG2 cells overexpressing a membrane transporter NTCP.利用过表达膜转运蛋白 NTCP 的 HepG2 细胞评价和鉴定乙型肝炎病毒进入抑制剂。
Biochem Biophys Res Commun. 2014 Jan 17;443(3):808-13. doi: 10.1016/j.bbrc.2013.12.052. Epub 2013 Dec 14.
8
E-cadherin Plays a Role in Hepatitis B Virus Entry Through Affecting Glycosylated Sodium-Taurocholate Cotransporting Polypeptide Distribution.E-钙黏蛋白通过影响糖基化牛磺胆酸钠共转运多肽的分布在乙型肝炎病毒进入中发挥作用。
Front Cell Infect Microbiol. 2020 Feb 27;10:74. doi: 10.3389/fcimb.2020.00074. eCollection 2020.
9
Molecular determinants of hepatitis B and D virus entry restriction in mouse sodium taurocholate cotransporting polypeptide.乙型肝炎和丁型肝炎病毒进入限制的分子决定因素在小鼠牛磺胆酸钠共转运多肽中。
J Virol. 2013 Jul;87(14):7977-91. doi: 10.1128/JVI.03540-12. Epub 2013 May 15.
10
HBV life cycle is restricted in mouse hepatocytes expressing human NTCP.乙肝病毒生命周期在表达人钠离子-牛磺胆酸共转运多肽（NTCP）的小鼠肝细胞中受到限制。
Cell Mol Immunol. 2014 Mar;11(2):175-83. doi: 10.1038/cmi.2013.66. Epub 2014 Feb 10.
引用本文的文献
1
Advances in deciphering the mechanisms of salt tolerance in Maize.玉米耐盐机制解析的进展
Plant Signal Behav. 2025 Dec;20(1):2479513. doi: 10.1080/15592324.2025.2479513. Epub 2025 Mar 18.
2
Identification of NTCP animal orthologs supporting hepatitis B virus binding and infection.支持乙型肝炎病毒结合与感染的NTCP动物直系同源物的鉴定。
J Virol. 2025 Apr 15;99(4):e0183324. doi: 10.1128/jvi.01833-24. Epub 2025 Mar 5.
3
Hepatitis B: Model Systems and Therapeutic Approaches.乙型肝炎：模型系统与治疗方法。
J Immunol Res. 2024 May 7;2024:4722047. doi: 10.1155/2024/4722047. eCollection 2024.
4
HBV cccDNA-A Culprit and Stumbling Block for the Hepatitis B Virus Infection: Its Presence in Hepatocytes Perplexed the Possible Mission for a Functional Cure.乙肝病毒共价闭合环状DNA——乙肝病毒感染的罪魁祸首与绊脚石：其在肝细胞中的存在使功能性治愈的可能使命陷入困境。
ACS Omega. 2022 Jul 7;7(28):24066-24081. doi: 10.1021/acsomega.2c02216. eCollection 2022 Jul 19.
5
HIV-1-Mediated Acceleration of Oncovirus-Related Non-AIDS-Defining Cancers.HIV-1介导的与肿瘤病毒相关的非艾滋病定义性癌症的加速发展
Biomedicines. 2022 Mar 25;10(4):768. doi: 10.3390/biomedicines10040768.
6
Elevated NTCP expression by an iPSC-derived human hepatocyte maintenance medium enhances HBV infection in NTCP-reconstituted HepG2 cells.由诱导多能干细胞衍生的人肝细胞维持培养基提高NTCP表达，增强了NTCP重组HepG2细胞中的HBV感染。
Cell Biosci. 2021 Jul 5;11(1):123. doi: 10.1186/s13578-021-00641-1.
7
Innovative HBV Animal Models Based on the Entry Receptor NTCP.基于乙肝病毒进入受体 NTCP 的创新型 HBV 动物模型。
Viruses. 2020 Jul 30;12(8):828. doi: 10.3390/v12080828.
8
Hepatitis B: Current Status of Therapy and Future Therapies.乙型肝炎：治疗现状与未来疗法。
Gastroenterol Clin North Am. 2020 Jun;49(2):215-238. doi: 10.1016/j.gtc.2020.01.003. Epub 2020 Mar 29.
9
Disease Pathways and Mechanisms of Potential Drug Targets.疾病途径与潜在药物靶点的机制
Clin Liver Dis (Hoboken). 2018 Aug 22;12(1):12-18. doi: 10.1002/cld.735. eCollection 2018 Jul.
10
A Single Adaptive Mutation in Sodium Taurocholate Cotransporting Polypeptide Induced by Hepadnaviruses Determines Virus Species Specificity.肝病毒诱导的钠牛磺胆酸共转运多肽中的单个适应性突变决定病毒种属特异性。
J Virol. 2019 Feb 19;93(5). doi: 10.1128/JVI.01432-18. Print 2019 Mar 1.
本文引用的文献
1
A conformational heparan sulfate binding site essential to infectivity overlaps with the conserved hepatitis B virus a-determinant.一个对感染性至关重要的构象性硫酸乙酰肝素结合位点与保守的乙型肝炎病毒 a 决定簇重叠。
Hepatology. 2013 Mar;57(3):985-94. doi: 10.1002/hep.26125. Epub 2013 Feb 15.
2
Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus.牛磺胆酸钠共转运多肽是乙型肝炎病毒和丁型肝炎病毒的功能性受体。
Elife. 2012 Nov 13;1:e00049. doi: 10.7554/eLife.00049.
3
Dating the origin and dispersal of hepatitis B virus infection in humans and primates.追溯乙型肝炎病毒在人类和灵长类动物中的感染起源和传播。
Hepatology. 2013 Mar;57(3):908-16. doi: 10.1002/hep.26079. Epub 2013 Feb 7.
4
Global epidemiology of hepatitis B virus infection: new estimates of age-specific HBsAg seroprevalence and endemicity.全球乙型肝炎病毒感染流行病学：特定年龄 HBsAg 血清流行率和地方性的新估计。
Vaccine. 2012 Mar 9;30(12):2212-9. doi: 10.1016/j.vaccine.2011.12.116. Epub 2012 Jan 24.
5
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.细菌胆汁酸钠协同转运蛋白 ASBT 同源物的晶体结构
Nature. 2011 Oct 5;478(7369):408-11. doi: 10.1038/nature10450.
6
The role of the sodium-taurocholate cotransporting polypeptide (NTCP) and of the bile salt export pump (BSEP) in physiology and pathophysiology of bile formation.牛磺胆酸钠共转运多肽（NTCP）和胆盐输出泵（BSEP）在胆汁形成的生理和病理生理中的作用。
Handb Exp Pharmacol. 2011(201):205-59. doi: 10.1007/978-3-642-14541-4_5.
7
Fine mapping of pre-S sequence requirements for hepatitis B virus large envelope protein-mediated receptor interaction.乙型肝炎病毒大 envelope 蛋白介导的受体相互作用的前 S 序列要求的精细作图。
J Virol. 2010 Feb;84(4):1989-2000. doi: 10.1128/JVI.01902-09. Epub 2009 Dec 9.
8
The pre-S1 and antigenic loop infectivity determinants of the hepatitis B virus envelope proteins are functionally independent.乙型肝炎病毒包膜蛋白的前S1和抗原环感染性决定簇在功能上是独立的。
J Virol. 2009 Dec;83(23):12443-51. doi: 10.1128/JVI.01594-09. Epub 2009 Sep 16.
9
Prevention of hepatitis B virus infection in vivo by entry inhibitors derived from the large envelope protein.源自大包膜蛋白的进入抑制剂在体内预防乙型肝炎病毒感染
Nat Biotechnol. 2008 Mar;26(3):335-41. doi: 10.1038/nbt1389. Epub 2008 Feb 24.
10
Infectivity determinants of the hepatitis B virus pre-S domain are confined to the N-terminal 75 amino acid residues.乙肝病毒前S结构域的感染性决定因素局限于N端的75个氨基酸残基。
J Virol. 2007 Jun;81(11):5841-9. doi: 10.1128/JVI.00096-07. Epub 2007 Mar 21.
Zotero 插件