Fang Yuehui, Zhou Yiting, Zhong Yan, Gao Xin, Tan Tao
Department of Nutrition, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China.
Wei Sheng Yan Jiu. 2013 Jan;42(1):18-22.
To study the effects of bisphenol A (BPA) on reproductive functions and oxidative stress in adolescent male mice, and to explore the effect of high dose vitamin E on reproductive functions and oxidative stress induced by BPA.
Thirty-two kunming male mice aged 4 -5 weeks were randomly divided into 3 groups: control group (gavaged with corn oil 0.2 ml/d, n = 10), BPA group (gavaged with BPA 0.5 mg/kg BW, n = 11), and vitamin E intervention group (gavaged with BPA 0.5 mg/kg BW and vitamin E 150mg/kg BW, n = 11). All animals were sacrificed 3 weeks later and blood and tissue samples were collected.
The wet weight of testis in BPA group, the wet weight and organ coefficient of spermatophore, and counts of sperm in BPA and vitamin E intervention groups were significantly lower than those in the control group (P < 0.05 or P < 0.01), and the organ coefficient of spermatophore in vitamin E intervention group was significantly higher than BPA group (P < 0.05). The rates of teratosperm in BPA and vitamin E intervention groups were significant higher than the control group (P < 0.05), and the activity ratio of sperm in BPA group was significantly lower than those in the control and vitamin E intervention groups (P < 0.05). The SOD activities of liver tissue in both BPA and vitamin E intervention groups and CAT activity in BPA group were significantly higher than the control group (P < 0.05). The serum testosterone level in BPA group was lower than the control and vitamin E intervention group without significant difference.
Short-term BPA exposure may partly inhibit the reproductive function in adolescent male mice with certain stimulating effect on antioxidant ability, and supplementation of vitamin E during BPA exposure may have certain protective effect on reproductive inhibition caused by BPA exposure.
研究双酚A（BPA）对青春期雄性小鼠生殖功能和氧化应激的影响，并探讨高剂量维生素E对BPA诱导的生殖功能和氧化应激的影响。
将32只4-5周龄的昆明雄性小鼠随机分为3组：对照组（每天灌胃0.2 ml玉米油，n = 10）、BPA组（每天灌胃0.5 mg/kg体重的BPA，n = 11）和维生素E干预组（每天灌胃0.5 mg/kg体重的BPA和150 mg/kg体重的维生素E，n = 11）。3周后处死所有动物，采集血液和组织样本。
BPA组睾丸湿重、精囊湿重和器官系数以及BPA组和维生素E干预组的精子计数均显著低于对照组（P < 0.05或P < 0.01），维生素E干预组精囊器官系数显著高于BPA组（P < 0.05）。BPA组和维生素E干预组的畸形精子率显著高于对照组（P < 0.05），BPA组精子活动率显著低于对照组和维生素E干预组（P < 0.05）。BPA组和维生素E干预组肝组织超氧化物歧化酶（SOD）活性以及BPA组过氧化氢酶（CAT）活性均显著高于对照组（P < 0.05）。BPA组血清睾酮水平低于对照组和维生素E干预组，但差异无统计学意义。
短期暴露于BPA可能部分抑制青春期雄性小鼠的生殖功能，对抗氧化能力有一定刺激作用，BPA暴露期间补充维生素E可能对BPA暴露引起的生殖抑制有一定保护作用。
