碲化镉量子点对小鼠肝脏的氧化损伤作用

[Oxidative damage effects induced by CdTe quantum dots in mice liver].

作者信息

Xie Guangyun, Du Qingcheng, Zheng Min, Chen Wei, Liu Na, Lu Yang, Huang Peili, Sun Zhiwei

机构信息

Insititue for Ocuupational Health and Poisoning Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

出版信息

Wei Sheng Yan Jiu. 2013 Jan;42(1):39-43.

PMID:23596705

Abstract

OBJECTIVE

To investigate oxidative damage effects induced by CdTe quantum dots (QDs) in mice liver.

METHODS

40 ICR mice were randomly divided into 5 groups, one control group (normal saline), four CdTe QDs (exposed by intravenous injection of 0.2 ml CdTe QDs at the concentration of 0, 0.5, 5, 50 and 500 nmol/ml respectively). All mice were decapitated 24h after the injection. Concentration of MDA and the activities of SOD, CAT, 8-OHdG expression were examined by immunohistochemistry and hepatocellular apoptosis was measured with TUNEL resperctively.

RESULTS

The results showed in 500 nmol/ml CdTe QDs group, MDA concentration in the liver tissue were significantly higher than those of control group (P < 0.05). The activities of SOD in 50 and 500 nmol/ml and CAT in 5, 50 and 500 nmol/ml CdTe QDs groups were significantly lower than those of control group (P < 0.01). Positive staining for 8-OHdG appeared in 50 and 500 nmol/ml CdTe QDs groups and the apoptosis of liver cells was observed in 500 nmol/ml CdTe QDs group by TUNEL technique.

CONCLUSION

It was suggested that CdTe QDs at certain doses could induce oxidative damage effects of the mice liver in the condition of this experiment.

摘要

目的

探讨碲化镉量子点（QDs）对小鼠肝脏的氧化损伤作用。

方法

将40只ICR小鼠随机分为5组，1个对照组（生理盐水），4个碲化镉量子点组（分别经静脉注射浓度为0、0.5、5、50和500 nmol/ml的碲化镉量子点0.2 ml）。注射后24小时将所有小鼠断头。采用免疫组织化学法检测丙二醛（MDA）浓度、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性及8-羟基脱氧鸟苷（8-OHdG）表达，采用末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）检测肝细胞凋亡情况。

结果

结果显示，在500 nmol/ml碲化镉量子点组中，肝组织中MDA浓度显著高于对照组（P < 0.05）。50和500 nmol/ml碲化镉量子点组的SOD活性以及5、50和500 nmol/ml碲化镉量子点组的CAT活性均显著低于对照组（P < 0.01）。50和500 nmol/ml碲化镉量子点组出现8-OHdG阳性染色，且在500 nmol/ml碲化镉量子点组通过TUNEL技术观察到肝细胞凋亡。