Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.
Talanta. 2013 Feb 15;105:52-6. doi: 10.1016/j.talanta.2012.11.053. Epub 2012 Dec 1.
In this work, an automated liquid operation method for multistep heterogeneous immunoassay toward point of care testing (POCT) was proposed. A miniaturized peristaltic pump was developed to control the flow direction, flow time and flow rate in the microliter range according to a program. The peristaltic pump has the advantages of simple structure, small size, low cost, and easy to build and use. By coupling the peristaltic pump with an antibody-coated capillary and a reagent-preloaded cartridge, the complicated liquid handling operation for heterogeneous immunoassay, including sample metering and introduction, multistep reagent introduction and rinsing, could be triggered by an action and accomplished automatically in 12 min. The analytical performance of the present immunoassay system was demonstrated in the measurement of human IgG with fluorescence detection. A detection limit of 0.68 μg/mL IgG and a dynamic range of 2-300 μg/mL were obtained.
在这项工作中，提出了一种用于即时检测（POCT）的多步异质免疫分析的自动化液体操作方法。 开发了一种微型蠕动泵，可根据程序控制微升范围内的流向、流动时间和流速。 蠕动泵具有结构简单、体积小、成本低、易于构建和使用等优点。 通过将蠕动泵与涂有抗体的毛细管和预加载试剂的试剂盒结合使用，可实现复杂的液体处理操作，包括样品计量和引入、多步试剂引入和冲洗，所有操作仅需一步触发即可自动完成，整个过程耗时 12 分钟。 荧光检测法用于测定人 IgG，验证了本免疫分析系统的分析性能。 得到的检测限为 0.68 μg/mL IgG，动态范围为 2-300 μg/mL。
