用于自动化快速免疫测定的一次性微流控芯片设计评估
Evaluation of disposable microfluidic chip design for automated and fast Immunoassays.
作者信息
Wang Guochun, Das Champak, Ledden Bradley, Sun Qian, Nguyen Chien, Kumar Sai
机构信息
SFC Fluidics, Inc. , Fayetteville, Arkansas 72701, USA.
Diligent CXO , Norcross, Georgia 30071, USA.
出版信息
Biomicrofluidics. 2017 Feb 22;11(1):014115. doi: 10.1063/1.4977198. eCollection 2017 Jan.
Abstract
We report here, the design and development of a disposable immunoassay chip for protein biomarker detection within ∼1 h. The unique design allows for real-time dynamic calibration of immunoassay for multiple biomarker detections on the chip. The limit of detection achieved for this test chip is 10 pg/ml for IL6, and 50 pg/ml for GFAP with a detection time of 1 h. The prototype instrument used for flowing the reagents through the chip can be easily assembled from off-the-shelf components with the final chemiluminescent detection carried out in a commercial plate reader. Optimization of different aspects of chip design, fabrication, and assay development is discussed in detail.
摘要
我们在此报告一种用于在约1小时内检测蛋白质生物标志物的一次性免疫分析芯片的设计与开发。独特的设计允许对芯片上多种生物标志物检测的免疫分析进行实时动态校准。该测试芯片对IL6的检测限为10 pg/ml，对GFAP的检测限为50 pg/ml，检测时间为1小时。用于使试剂流过芯片的原型仪器可以很容易地用现成的组件组装而成，最终的化学发光检测在商用酶标仪中进行。详细讨论了芯片设计、制造和分析开发等不同方面的优化。
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