1Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
Reprod Sci. 2013 Nov;20(11):1356-64. doi: 10.1177/1933719113485291. Epub 2013 Apr 18.
The aim of this study was to investigate the effect of neoadjuvant chemotherapy (NAC) on the human squamous cervical cancer using proteomics profiling and to obtain related proteins to NAC exposure and response.
Paired samples of early-stage bulky squamous cervical cancer before and after NAC treatment from patients who responded to NAC were obtained and submitted to 2-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS). The expression and localization of the interesting proteins in additional paired samples were confirmed by Western blot analysis and immunohistochemistry.
The comparison of the proteins present before and after NAC revealed that 116 protein spots were significantly changed. In all, 31 proteins were analyzed by MS, and 15 proteins were upregulated in the cancer tissue after NAC relative to the level before NAC, whereas 16 proteins were downregulated after NAC. The significantly higher expression of peroxiredoxin 1 and significantly lower expression of galectin 1 after NAC treatment were confirmed by Western blot.
Proteomics can be used to identify the NAC-related proteins in squamous cervical cancer. The change in proteins may be associated with NAC exposure and response, but insight into their relevance requires further study.
本研究旨在通过蛋白质组学分析探讨新辅助化疗（NAC）对人宫颈鳞癌的作用，并获得与 NAC 暴露和反应相关的蛋白。
收集对 NAC 治疗有反应的早期宫颈鳞癌患者 NAC 治疗前后的配对样本，进行二维凝胶电泳和基质辅助激光解吸/电离飞行时间质谱（MS）分析。通过 Western blot 分析和免疫组织化学验证了额外配对样本中感兴趣蛋白的表达和定位。
NAC 前后蛋白质的比较显示，有 116 个蛋白点发生了显著变化。通过 MS 分析了 31 种蛋白质，与 NAC 前相比，NAC 后癌组织中有 15 种蛋白上调，16 种蛋白下调。Western blot 进一步证实了 NAC 治疗后过氧化物酶 1 的表达显著上调，半乳糖凝集素 1 的表达显著下调。
蛋白质组学可用于鉴定宫颈鳞癌中与 NAC 相关的蛋白。蛋白的变化可能与 NAC 暴露和反应有关，但深入了解其相关性还需要进一步研究。
