Yamashita Y, Pedersen J H, Hansen C P
Dept. of Surgery C, Rigshospitalet, University of Copenhagen, Denmark.
Scand J Gastroenterol. 1990 May;25(5):481-8. doi: 10.3109/00365529009095519.
The distribution of neurotensin-like immunoreactivity (NTLI) in porcine and human intestine was studied by extraction of mucosal and muscular layers of esophagus, stomach, duodenum, jejunum, ileum, and colon. NTLI was quantitated and characterized by radioimmunoassays and gel filtration chromatography. Porcine tissue was obtained in anesthetized animals (n = 6) and human tissue during surgery (n = 28). Concentrations of NTLI increased gradually from the distal esophagus to the ileum. Highest concentrations were found in 2.0 M acetic acid extracts of proximal ileal mucosa (150 (131-223) and 525 (500-729) pmol/g wet tissue, respectively (medians and interquartile range]. After acid extraction, concentrations of intact NT and COOH-terminal and NH2-terminal NTLI were similar, but in water concentrations of NH2-terminal NTLI were high and intact NT and COOH-terminal NTLI low. The distribution of NTLI was similar in the two species. Gel chromatography of ileal, jejunal, and duodenal mucosa indicated that in these tissues NTLI consisted primarily of intact NT. In antral mucosa COOH-terminal immunoreactivity different from NT was detected. The chemical identity is unknown, but it may represent precursor forms of NT.
通过提取猪和人的食管、胃、十二指肠、空肠、回肠及结肠的黏膜层和肌层，研究了神经降压素样免疫反应性（NTLI）在猪和人肠道中的分布情况。采用放射免疫分析法和凝胶过滤色谱法对NTLI进行定量和特性分析。猪组织取自麻醉动物（n = 6），人组织取自手术过程中（n = 28）。NTLI的浓度从食管远端到回肠逐渐升高。在回肠近端黏膜的2.0 M乙酸提取物中发现最高浓度（分别为150（131 - 223）和525（500 - 729）pmol/g湿组织，中位数和四分位间距）。酸提取后，完整NT以及NTLI的COOH末端和NH2末端的浓度相似，但在水中，NH2末端NTLI的浓度高，而完整NT和COOH末端NTLI的浓度低。两种物种中NTLI的分布相似。回肠、空肠和十二指肠黏膜的凝胶色谱分析表明，在这些组织中NTLI主要由完整的NT组成。在胃窦黏膜中检测到不同于NT的COOH末端免疫反应性。其化学性质未知，但可能代表NT的前体形式。
