Jiang Jian-Guo, Zhang Xi-Ru, Zhang Yi-Hua, Song Geng-Shen
Hebei Institute for Food and Drug Control, Shijiazhuang 050011, China.
Yao Xue Xue Bao. 2013 Jan;48(1):94-7.
This paper reports the establishment of a method for rapid identification 15 effective components of anti common cold medicine (paracetamol, aminophenazone, pseudoephedrine hydrochloride, methylephedrine hydrochloride, caffeine, amantadine hydrochloride, phenazone, guaifenesin, chlorphenamine maleate, dextromethorphen hydrobromide, diphenhydramine hydrochloride, promethazine hydrochloride, propyphenazone, benorilate and diclofenac sodium) with MRM by LC-MS/MS. The samples were extracted by methanol and were separated from a Altantis T3 column within 15 min with a gradient of acetonitrile-ammonium acetate (containing 0.25% glacial acetic acid), a tandem quadrupole mass spectrometer equipped with electrospray ionization source (ESI) was used in positive ion mode, and multiple reaction monitoring (MRM) was performed for qualitative analysis of these compounds. The minimum detectable quantity were 0.33-2.5 microg x kg(-1) of the 15 compounds. The method is simple, accurate and with good reproducibility for rapid identification many components in the same chromatographic condition, and provides a reference for qualitative analysis illegally added chemicals in anti common cold medicine.
本文报道了一种采用液相色谱-串联质谱（LC-MS/MS）的多反应监测（MRM）模式快速鉴定抗感冒药中15种有效成分（对乙酰氨基酚、氨基比林、盐酸伪麻黄碱、盐酸甲基麻黄碱、咖啡因、盐酸金刚烷胺、非那西丁、愈创甘油醚、马来酸氯苯那敏、氢溴酸右美沙芬、盐酸苯海拉明、盐酸异丙嗪、安乃近、贝诺酯和双氯芬酸钠）的方法。样品用甲醇提取，在15分钟内于Altantis T3柱上以乙腈-醋酸铵（含0.25%冰醋酸）梯度进行分离，采用配备电喷雾电离源（ESI）的串联四极杆质谱仪在正离子模式下进行检测，通过多反应监测（MRM）对这些化合物进行定性分析。15种化合物的最低检测量为0.33 - 2.5μg·kg⁻¹ 。该方法简单、准确，在同一色谱条件下对多种成分进行快速鉴定时具有良好的重现性，为抗感冒药中非法添加化学物质的定性分析提供了参考。
