Sun Ying, Zhao Heng-Wei, Ge Feng, Shi Lei, Liu Di-Qiu
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
Yao Xue Xue Bao. 2013 Jan;48(1):138-43.
PNS (Panax notoginseng saponins) is the main medical bioactive component in Panax notoginseng. The medical value of PNS cannot be extended because of its low production. With the deep study of saponins biosynthetic pathway, the control of PNS biosynthesis through metabolic engineering has gradually become possible. In this study, the Squalene synthase (SS) over-expression vector was established. By the way of agrobacterium-mediated method, the vector was transfered and integrated into the Panax notoginseng genome. The result of the PCR detection and the saponin content detection shows that over-expression SS is able to produce high level of Panax notoginseng saponins, and confirms the regulatory function of SS in the biosynthesis of ginsenosides in Panax notoginseng. It provides a theoretical basis and technical basis for the construction of PNS homologous or heterologous efficient expression system in the future.
三七总皂苷（PNS）是三七的主要药用生物活性成分。由于其产量较低，PNS的药用价值无法得到充分发挥。随着对皂苷生物合成途径的深入研究，通过代谢工程控制PNS生物合成逐渐成为可能。在本研究中，构建了鲨烯合酶（SS）过表达载体。通过农杆菌介导的方法，将该载体转入并整合到三七基因组中。PCR检测和皂苷含量检测结果表明，过表达SS能够产生高水平的三七皂苷，证实了SS在三七人参皂苷生物合成中的调控作用。这为今后构建PNS同源或异源高效表达系统提供了理论依据和技术基础。
