Bond R, Brooks H
Department of Veterinary Clinical Sciences, Royal Veterinary College, North Mymms, Hatfield, Herts, AL9 7TA.
J Small Anim Pract. 2013 Jun;54(6):299-303. doi: 10.1111/jsap.12067. Epub 2013 Apr 21.
To evaluate transverse sectioning for histological assessment of canine sebaceous glands in health and disease.
Skin specimens were obtained from the cadavers of six healthy beagle dogs and from an archive of tissue sections of 24 dogs with a histological diagnosis of sebaceous adenitis.
In healthy beagle dogs, both the mean numbers of compound hair follicles and sebaceous lobes observed in transverse sections were approximately threefold higher (P < 0. 001) than those seen in vertical sections. A similar relationship was noted in examples of sebaceous adenitis. In cases of sebaceous adenitis, there was a complete absence of sebaceous glands in paired transverse and vertical sections in 14 cases, whereas in 3 dogs glands were observed in transverse sections where none were seen in vertical sections. The percentages of hair follicles with associated sebaceous glands in affected dogs did not vary between transverse (12. 3%) and vertical (13 . 3%) sections, whereas 89% (vertical) and 96% (transverse) of follicles had associated glands in the healthy beagle dogs.
Transverse sectioning of canine skin specimens to allow visualization of the follicular isthmus or deep portion of the common infundibulum enhances the histological assessment of sebaceous glands in both health and disease.
评估横断面切片在健康和患病犬皮脂腺组织学评估中的应用。
从6只健康比格犬的尸体以及24只经组织学诊断为皮脂腺炎的犬的组织切片存档中获取皮肤标本。
在健康比格犬中，横断面上观察到的复合毛囊和皮脂腺叶的平均数量比纵断面上的大约高三倍（P < 0.001）。在皮脂腺炎病例中也观察到类似的关系。在皮脂腺炎病例中，14例的配对横断面和纵断面中完全没有皮脂腺，而在3只犬中，横断面上观察到有腺体，而纵断面上未观察到。患病犬中伴有皮脂腺的毛囊百分比在横断面（12.3%）和纵断面（13.3%）之间没有差异，而在健康比格犬中，89%（纵断面）和96%（横断面）的毛囊伴有腺体。
对犬皮肤标本进行横断面切片以观察毛囊峡部或共同漏斗深部，可增强对健康和患病状态下皮脂腺的组织学评估。
