血管紧张素转换酶基因多态性与糖尿病视网膜病变的关系

[Relationship of angiotensin converting enzyme gene polymorphism with diabetic retinopathy].

作者信息

Li Na, Yang Xiu-fen, Gu Hong, Deng Yu, Xu Jun, Ma Kai, Liu Ning-pu

机构信息

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Laboratory, Beijing 100730, China.

出版信息

Zhonghua Yan Ke Za Zhi. 2013 Jan;49(1):52-7.

PMID:23601467

Abstract

OBJECTIVE

To investigate the association between angiotensin converting enzyme (ACE) gene locus rs1799752 insertion/deletion (I/D) polymorphism and diabetic retinopathy (DR) in type 2 diabetes mellitus.

METHODS

Case-control study. Type 2 diabetes patients were recruited and assigned into DR group, which included proliferative diabetic retinopathy (PDR) group or diabetes without retinopathy (DWR) group. Volunteers without diabetes from the same community were recruited as the control group. PCR and agarose gel electrophoresis methods were adopted to determine the rs1799752 I/D polymorphism genotypes of the ACE gene. The frequency of genotypes and alleles was compared among the various groups.

RESULTS

Four hundred and twelve diabetes patients: (207 subjects of DR, including 53 subjects of PDR and 205 subjects of DWR) and 97 non-diabetic control subjects were included in the study. The frequencies of the I and D alleles of ACE rs1799752 polymorphism were 54.1% and 45.9%, respectively, in the DR group, 52.8% and 47.2% in the PDR group, and 48.0% and 52.0% in the DWR group. There were no statistical differences between DR and DWR groups (χ(2) = 3.02, P > 0.05) or between PDR and DWR groups (χ(2) = 0.77, P > 0.05). Moreover, there were no statistical differences in the distribution of the ACE genotypes between DR group (II 25.1%, ID 58.0%, DD 16.9%) and DWR group (II 22.0%, ID 52.2%, DD 25.9%) (χ(2) = 4.92, P > 0.05) or between PDR group (II 20.7%, ID 64.2%, DD 15.1%) and DWR group (χ(2) = 3.19, P > 0.05). No statistical differences were found in the frequencies of the I and D alleles, and the distributions of I/D genotypes between diabetic group and the control group (χ(2) = 0.25, 4.98; P > 0.05). In the multiple regressions model including clinical factors such as the age of onset of diabetes, urinary albumin, insulin usage, creatinine, glycated hemoglobin, fast glucose, and the use of ACE inhibitor, no association was found between ACE gene polymorphism and DR (OR = 0.80, 95%CI: 0.59 - 1.09) or PDR (OR = 1.23, 95%CI: 0.78 - 1.93).

CONCLUSION

There is no association between ACE rs1799752 gene insertion/deletion (I/D) polymorphism and DR in patients with type 2 diabetes mellitus.

摘要

目的

探讨血管紧张素转换酶（ACE）基因位点rs1799752插入/缺失（I/D）多态性与2型糖尿病患者糖尿病视网膜病变（DR）之间的关联。

方法

病例对照研究。招募2型糖尿病患者并分为DR组，其中包括增殖性糖尿病视网膜病变（PDR）组和无糖尿病视网膜病变（DWR）组。招募来自同一社区的无糖尿病志愿者作为对照组。采用聚合酶链反应（PCR）和琼脂糖凝胶电泳方法确定ACE基因的rs1799752 I/D多态性基因型。比较各组基因型和等位基因频率。

结果

本研究纳入412例糖尿病患者（207例DR患者，包括53例PDR患者和205例DWR患者）和97例非糖尿病对照者。DR组中ACE rs1799752多态性的I和D等位基因频率分别为54.1%和45.9%，PDR组为52.8%和47.2%，DWR组为48.0%和52.0%。DR组与DWR组之间（χ(2)=3.02，P＞0.05）或PDR组与DWR组之间（χ(2)=0.77，P＞0.05）无统计学差异。此外，DR组（II 25.1%，ID 58.0%，DD 16.9%）与DWR组（II 22.0%，ID 52.2%，DD 25.9%）之间（χ(2)=4.92，P＞0.05）或PDR组（II 20.7%，ID 64.2%，DD 15.1%）与DWR组之间（χ(2)=3.19，P＞0.05）ACE基因型分布无统计学差异。糖尿病组与对照组之间I和D等位基因频率及I/D基因型分布无统计学差异（χ(2)=0.25，4.98；P＞0.05）。在包括糖尿病发病年龄、尿白蛋白、胰岛素使用、肌酐、糖化血红蛋白、空腹血糖和ACE抑制剂使用等临床因素的多元回归模型中，未发现ACE基因多态性与DR（比值比[OR]=0.80，95%置信区间[CI]：0.59 - 1.09）或PDR（OR = 1.23，95%CI：0.78 - 1.93）之间存在关联。