Zhang Lei, Zhang Hong, Song Guo-xiang, Lin Ting-ting, Xu Guang-chang, Zhu Li-min
Tianjin Eye Hospital, Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin Eye Institute, the Ophthalmic College of Tianjin Medical University, Tianjin 300020, China.
Zhonghua Yan Ke Za Zhi. 2013 Jan;49(1):58-63.
To investigate the expression of matrix metal proteinase (MMP)-2 and MMP-9 in adenoid cystic carcinoma of lacrimal gland as well as their relation with biological behaviour of adenoid cystic carcinoma.
Experimental study. The research objects were 60 cases of adenoid cystic carcinoma of lacrimal gland which were collected from No.2 Hospital of Tianjin Medical University from January 1991 to Jule 2011. There were 25 males and 35 females aged from 29 to 42 years. Based on histological revision, there were 36 cases of cribriform-tubular subtype and 24 cases of solid subtype. Forty-five cases were primary lesions and 15 cases were recurrent lesions. Ten samples of normal lacrimal gland around polymorphic adenoma were selected as the control group. The expression of CD105, MMP-2 and MMP-9 were evaluated by immunohistochemistry. The microvessel density (MVD) was defined by expression of CD105. One way ANOVA, χ(2)-test and spearman correlation test were used to analyzed the data.
The number of MVD [(17.71 ± 5.63)/100 folds field of vision] and the positive rates of MMP-2 (45.0%, 27/60) and MMP-9 (55.0%, 33/60) in the samples of adenoid cystic carcinoma of lacrimal gland were higher than those in the normal lacrimal gland [the number of MVD was (0.70 ± 0.95)/100 folds field of vision, the expressions of MMP-2 and MMP-9 were negative] (t' = 2.039, P < 0.05; χ(2) = 5.550, P < 0.05; χ(2) = 8.315, P < 0.01), the solid subtypes had more MVD [(26.12 ± 5.32)/100 folds field of vision] and higher positive rates of MMP-2 (62.5%, 15/24) and MMP-9 (79.2%, 19/24) than the cribriform-tubular subtypes (t' = 2.060, P < 0.05; χ(2) = 4.950, P < 0.05; χ(2) = 9.439, P < 0.05); the recurrent lesions had more MVD and higher positive rate of MMP-2 and MMP-9 than the primary lesions (t' = 2.129, P < 0.05; χ(2) = 9.899, P < 0.05; χ(2) = 8.103, P < 0.05). The number of MVD in ACC of lacrimal gland patients was correlated with the positive rate of MMP-2 and MMP-9 respectively (rs = 0.636, P < 0.05; rs = 0.524, P < 0.05).
The number of MVD and the expression of MMP-2 and MMP-9 are higher level in adenoid cystic carcinoma of lacrimal gland and are significantly correlated with pathological type and recurrence. Detecting the number of MVD and the expression of MMP-2 and MMP-9 may become biological indexes for malignancy, recurrence and metastasis of adenoid cystic carcinoma of lacrimal gland.
探讨基质金属蛋白酶（MMP）-2和MMP-9在泪腺腺样囊性癌中的表达及其与腺样囊性癌生物学行为的关系。
实验研究。研究对象为1991年1月至2011年7月天津医科大学第二医院收集的60例泪腺腺样囊性癌患者。其中男性25例，女性35例，年龄29～42岁。根据组织学分类，筛状-管状型36例，实体型24例。原发灶45例，复发灶15例。选取多形性腺瘤周围正常泪腺组织10例作为对照组。采用免疫组织化学法检测CD105、MMP-2和MMP-9的表达。以CD105的表达定义微血管密度（MVD）。采用单因素方差分析、χ²检验和Spearman相关检验进行数据分析。
泪腺腺样囊性癌组织中MVD数量[（17.71±5.63）/100倍视野]、MMP-2阳性率（45.0%，27/60）和MMP-9阳性率（55.0%，33/60）均高于正常泪腺组织[MVD数量为（0.70±0.95）/100倍视野，MMP-2和MMP-9表达均为阴性]（t'=2.039，P<0.05；χ²=5.550，P<0.05；χ²=8.315，P<0.01）；实体型MVD数量[（26.12±5.32）/100倍视野]、MMP-2阳性率（62.5%，15/24）和MMP-9阳性率（79.2%，19/24）均高于筛状-管状型（t'=2.060，P<0.05；χ²=4.950，P<0.05；χ²=9.439，P<0.05）；复发灶MVD数量、MMP-2和MMP-9阳性率均高于原发灶（t'=2.129，P<0.05；χ²=9.899，P<0.05；χ²=8.103，P<0.05）。泪腺腺样囊性癌患者MVD数量分别与MMP-2、MMP-9阳性率呈正相关（rs=0.636，P<0.05；rs=0.524，P<0.05）。
泪腺腺样囊性癌中MVD数量及MMP-2和MMP-9表达均处于较高水平，且与病理类型及复发密切相关。检测MVD数量及MMP-2和MMP-9表达可能成为泪腺腺样囊性癌恶性程度、复发及转移的生物学指标。
