Mishev Kiril, Dejonghe Wim, Russinova Eugenia
Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium.
Chem Biol. 2013 Apr 18;20(4):475-86. doi: 10.1016/j.chembiol.2013.03.009.
Endomembrane trafficking has a key role for ensuring homeostasis, growth and development, hormonal signaling, and adaptation of eukaryotes to the constantly changing environmental conditions. The complex organization of the endomembrane system implies the need for searching novel tools to specifically probe the regulatory components and dissect the tightly interconnected vesicle transport pathways. Here, we review the large-scale chemical genetic screens, which led to the identification of small molecules with an impact on various parts of the vesicle trafficking network. We discuss the similarities and differences in the organization of the endomembrane systems in yeasts, mammals, and plants based on studies of small molecules and their effects on trafficking hubs, routes, and conserved protein targets.
内膜运输对于确保真核生物的内环境稳定、生长发育、激素信号传导以及适应不断变化的环境条件起着关键作用。内膜系统的复杂组织意味着需要寻找新的工具来特异性地探测调控成分，并剖析紧密相连的囊泡运输途径。在这里，我们回顾了大规模化学遗传学筛选，这些筛选导致了对囊泡运输网络各个部分有影响的小分子的鉴定。基于对小分子及其对运输枢纽、途径和保守蛋白靶点的影响的研究，我们讨论了酵母、哺乳动物和植物内膜系统组织中的异同。
