Norambuena Lorena, Hicks Glenn R, Raikhel Natasha V
Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California Riverside, CA, USA.
Methods Mol Biol. 2009;495:133-43. doi: 10.1007/978-1-59745-477-3_12.
Plant endomembrane system is essential for viability and necessary for proper development and signal transduction signal processes. Links between the endomembrane system and auxin signaling have been reported by classical genetics screens. However, the relationship between these processes is not well understood. Chemical genomics is a powerful approach to dissect various processes overcoming lethality and redundancy issues. This approach uses small molecules to modify or disrupt the function of specific proteins and biological processes. We present a screen in Arabidopsis thaliana to identify compound affecting auxin-dependent responses and components of the endomembrane system. A gravitropic-response based screen is performed in Arabidopsis seedlings. The identified gravitropic effectors are tested in terms of auxin responsiveness and their effects on endomembrane compartments. These bioactive compounds will be valuable tools for dissecting endomembrane trafficking and auxin signaling processes.
植物内膜系统对于细胞存活至关重要，对于正常发育和信号转导过程也是必需的。经典遗传学筛选已报道了内膜系统与生长素信号传导之间的联系。然而，这些过程之间的关系尚未得到充分理解。化学基因组学是一种剖析各种过程的有力方法，可克服致死性和冗余性问题。该方法使用小分子来修饰或破坏特定蛋白质和生物过程的功能。我们在拟南芥中进行了一项筛选，以鉴定影响生长素依赖性反应和内膜系统成分的化合物。在拟南芥幼苗中进行了基于向重力性反应的筛选。对鉴定出的向重力性效应物进行生长素反应性及其对内膜区室影响方面的测试。这些生物活性化合物将成为剖析内膜运输和生长素信号传导过程的有价值工具。
