Robinson Nicholas A, Manivel J Carlos, Olson Erik J
Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, College of Veterinary Medicine, 244 Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA.
J Vet Diagn Invest. 2013 May;25(3):447-52. doi: 10.1177/1040638713482918. Epub 2013 Apr 19.
Mixed germ cell tumors of the ovary have rarely been reported in veterinary species. A 3-year-old intact female Labrador Retriever dog was presented for lethargy, abdominal distention, and a midabdominal mass. An exploratory laparotomy revealed a large (23 cm in diameter) left ovarian tumor and multiple small (2-3 cm in diameter) pale tan masses on the peritoneum and abdominal surface of the diaphragm. Histological examination of the left ovary revealed a mixed germ cell tumor with a yolk sac component with rare Schiller-Duval bodies and a teratomatous component comprised primarily of neural differentiation. The abdominal metastases were solely comprised of the yolk sac component. The yolk sac component was diffusely immunopositive for cytokeratin with scattered cells reactive for α-fetoprotein and placental alkaline phosphatase. Within the teratomatous component, the neuropil was diffusely immunopositive for S100, neuron-specific enolase, and neurofilaments with a few glial fibrillary acidic protein immunopositive cells. Ovarian germ cell tumors may be pure and consist of only 1 germ cell element or may be mixed and include more than 1 germ cell element, such as teratoma and yolk sac tumor.
卵巢混合性生殖细胞肿瘤在兽医临床物种中鲜有报道。一只3岁未绝育的雌性拉布拉多猎犬因嗜睡、腹部膨隆和腹部肿物前来就诊。剖腹探查发现一个巨大的（直径23厘米）左侧卵巢肿瘤，以及在腹膜和膈肌腹面有多个小的（直径2 - 3厘米）淡褐色肿物。左侧卵巢的组织学检查显示为一个混合性生殖细胞肿瘤，伴有卵黄囊成分，罕见席勒-杜瓦尔小体，畸胎瘤成分主要由神经分化构成。腹部转移灶仅由卵黄囊成分构成。卵黄囊成分对细胞角蛋白呈弥漫性免疫阳性，散在细胞对甲胎蛋白和胎盘碱性磷酸酶呈反应性。在畸胎瘤成分内，神经纤维网对S100、神经元特异性烯醇化酶和神经丝呈弥漫性免疫阳性，有少数胶质纤维酸性蛋白免疫阳性细胞。卵巢生殖细胞肿瘤可以是单纯性的，仅由一种生殖细胞成分组成，也可以是混合性的，包括不止一种生殖细胞成分，如畸胎瘤和卵黄囊瘤。
