Song Erxi, Wang Meizhen, Shen Dongsheng
College of Environmental Science and Engineering, Zhejiang Gongshang University, 198 Jiaogong Rd, Hangzhou, Zhejiang, China,
Biodegradation. 2014 Feb;25(1):85-94. doi: 10.1007/s10532-013-9642-5. Epub 2013 Apr 21.
A gram-negative strain, designated as FD-1, isolated from aerobic activated sludge was capable of metabolizing 4-fluoroaniline (4-FA) as its sole carbon and nitrogen source and energy supply. According to the Biolog GNIII detection method 17 of 71 carbon substrates were easily utilized, while 12 of 23 substrates did not inhibit strain FD-1. The 16S rDNA sequence from strain FD-1 was 99 % similar to Ralstonia sp., suggesting that it belonged to the genus Ralstonia. The optimal conditions for growth and 4-FA degradation were pH 7 and 30 °C. The tolerance to 4-FA were 1,250 mg/L, while the tolerance to salinity was 15 g/L. Catechol 2,3-dioxygenase activity was detected and degradation intermediates were analyzed by liquid chromatography mass spectrometry leading to a proposed degradation pathway and suggesting that extradiol cleavage was involved in 4-FA degradation. This is the first report on the degradation of 4-FA by a bacterium from the Ralstonia genus.
从好氧活性污泥中分离出的一株革兰氏阴性菌，命名为FD-1，能够以4-氟苯胺（4-FA）作为唯一的碳源、氮源和能量来源进行代谢。根据Biolog GNIII检测方法，71种碳源底物中有17种易于利用，而23种底物中有12种对菌株FD-1没有抑制作用。菌株FD-1的16S rDNA序列与拉尔斯顿菌属（Ralstonia sp.）相似度为99%，表明它属于拉尔斯顿菌属。生长和4-FA降解的最佳条件是pH 7和30℃。对4-FA的耐受浓度为1250 mg/L，对盐度的耐受浓度为15 g/L。检测到儿茶酚2,3-双加氧酶活性，并通过液相色谱-质谱联用分析降解中间体，从而提出了一条降解途径，表明4-FA降解过程涉及间位裂解。这是关于拉尔斯顿菌属细菌降解4-FA的首次报道。
