Zhu Shun-Ni, Liu Dong-Qi, Fan Li, Ni Jin-Ren
The Key Laboratory of Environmental and Urban Sciences, Shenzhen Graduate School of Peking University, Shenzhen 518055, PR China.
J Hazard Mater. 2008 Dec 30;160(2-3):289-94. doi: 10.1016/j.jhazmat.2008.02.112. Epub 2008 Mar 8.
A novel aerobic gram-positive bacterial strain capable of utilizing quinoline as sole source of carbon, nitrogen and energy was isolated from activated sludge of a coke plant wastewater treatment process. The isolate was identified as Rhodococcus sp. QL2 based on its morphology, physiochemical properties in addition to the results from 16S rDNA sequence analysis. The optimum temperature and the pH for its growth were 35-40 degrees C and 8.0, respectively. Extra nitrogen sources stimulated the bacterial growth on quinoline. Strain QL2 had strong quinoline degradability, and its degradation kinetics could be described with Haldane's model. Strain QL2 also had a broad range of substrate utilization. Identification of intermediates by GC/MS showed Rhodococcus sp. QL2 degraded quinoline via two pathways simultaneously.
从某焦化厂废水处理工艺的活性污泥中分离出一种新型需氧革兰氏阳性细菌菌株，该菌株能够将喹啉作为唯一的碳、氮和能量来源加以利用。除了16S rDNA序列分析结果外，根据其形态和理化性质，该分离菌株被鉴定为红球菌属QL2。其生长的最适温度和pH分别为35-40℃和8.0。额外的氮源促进了该细菌在喹啉上的生长。QL2菌株具有很强的喹啉降解能力，其降解动力学可用霍尔丹模型描述。QL2菌株还具有广泛的底物利用范围。通过气相色谱/质谱联用仪对中间产物进行鉴定表明，红球菌属QL2通过两条途径同时降解喹啉。
