在离体灌流大鼠肝中对真菌毒素脱氧雪腐镰刀菌烯醇的代谢研究。

On the metabolism of the mycotoxin deoxynivalenol in the isolated perfused rat liver.

机构信息

Institute for Medical Microbiology, Infectious and Epidemic Diseases Faculty of Veterinary Medicine, University of Munich, Veterinärstrasse 13, D-8000, Munich 22, FRG.

出版信息

Mycotoxin Res. 1987 Mar;3(1):25-32. doi: 10.1007/BF03191982.

DOI:10.1007/BF03191982

PMID:23604810

Abstract

An isolated rat liver was perfused with deoxynivalenol (DON) at a dose of 3 mg in a recirculating perfusion system. To identify glucuronide conjugates equal amounts of bile samples, perfusate and liver homogenates were incubated with and without (control) a β-glucuronidase preparation and analyzed by thin layer chromatography and capillary gas liquid chromatography - chemical ionization mass spectrometry. A total of 40.4% of the administered dose of DON was found to be conjugated with glucuronic acid (perfusate 20.4%, bile 19.2%, liver 0.8%), while only 1.3% of the parent DON (perfusate 1.1%, bile 0.2%) was detected. The cleavage of DON-glucuronide was demonstrated by incubating DON-glucuronide containing bile samples with intestine contents under anaerobic conditions.

摘要

在一个再循环灌注系统中，用 3 毫克的脱氧雪腐镰刀菌烯醇（DON）对离体大鼠肝脏进行灌注。为了鉴定葡萄糖醛酸结合物，等量的胆汁样品、灌流液和肝匀浆与（对照）β-葡萄糖醛酸酶制剂孵育，并通过薄层色谱法和毛细管气相色谱-化学电离质谱法进行分析。发现给予的 DON 剂量中有 40.4%与葡萄糖醛酸结合（灌流液 20.4%，胆汁 19.2%，肝脏 0.8%），而只有 1.3%的母体 DON（灌流液 1.1%，胆汁 0.2%）被检测到。通过在厌氧条件下用肠内容物孵育含有 DON-葡萄糖醛酸结合物的胆汁样品，证明了 DON-葡萄糖醛酸结合物的裂解。