Gareis M, Hashem A, Bauer J, Gedek B
Toxicol Appl Pharmacol. 1986 Jun 15;84(1):168-72. doi: 10.1016/0041-008x(86)90424-2.
Isolated rat livers were perfused with either 2 mg T-2 toxin or diacetoxyscirpenol (DAS) in a recirculating perfusion system. To identify glucuronide conjugates, equal amounts of bile samples were incubated with and without (control) a beta-glucuronidase preparation and analyzed by capillary gas liquid chromatography-chemical ionization mass spectrometry. Enzyme treatment of bile obtained from liver perfused with T-2 toxin resulted in the detection of a total of 954 micrograms HT-2 toxin (control 6 micrograms), demonstrating that excretion into the bile was mainly as glucuronide conjugates. Minor metabolites of T-2 toxin in bile were identified as 3'-hydroxy HT-2 toxin (TC-3), 3'-hydroxy-7-hydroxy HT-2 toxin (TC-6), and the glucuronide form of T-2 triol (trace amount). The glucuronide conjugates of monoacetoxyscirpenol (340 micrograms) and scirpenetriol (10 micrograms) were found in bile obtained from liver perfused with DAS, while nonconjugated metabolites were not detected. It is assumed that considerable amounts of T-2 toxin and DAS were metabolized biphasically. In phase I both trichothecenes were deacetylated, in phase II the metabolites were conjugated giving rise to the glucuronic acid adducts.
在循环灌注系统中，用2毫克T-2毒素或二乙酰氧基雪腐镰刀菌烯醇（DAS）灌注离体大鼠肝脏。为了鉴定葡萄糖醛酸共轭物，将等量的胆汁样品分别与β-葡萄糖醛酸酶制剂一起孵育（实验组）和不与该制剂孵育（对照组），然后通过毛细管气相色谱-化学电离质谱法进行分析。对用T-2毒素灌注肝脏所获得的胆汁进行酶处理后，共检测到954微克HT-2毒素（对照组为6微克），这表明排泄到胆汁中的主要是葡萄糖醛酸共轭物。胆汁中T-2毒素的次要代谢产物被鉴定为3'-羟基HT-2毒素（TC-3）、3'-羟基-7-羟基HT-2毒素（TC-6）以及T-2三醇的葡萄糖醛酸形式（痕量）。在用DAS灌注肝脏所获得的胆汁中发现了单乙酰氧基雪腐镰刀菌烯醇（340微克）和雪腐三醇（10微克）的葡萄糖醛酸共轭物，而未检测到非共轭代谢产物。据推测，相当数量的T-2毒素和DAS进行了双相代谢。在第一阶段，两种单端孢霉烯族毒素都发生了脱乙酰化，在第二阶段，代谢产物发生了共轭反应，生成了葡萄糖醛酸加合物。
