抗呋咱甲素 A 抗体的制备与鉴定。

Production and characterization of antibodies against fumigaclavine A.

机构信息

Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia,

出版信息

Mycotoxin Res. 2009 Oct;25(3):159-64. doi: 10.1007/s12550-009-0024-0. Epub 2009 Sep 12.

DOI:10.1007/s12550-009-0024-0

PMID:23605095

Abstract

Polyclonal antibodies against fumigaclavine A (FuA) were obtained from rabbits after immunization with a FuA-keyhole limpet hemocyanine conjugate prepared by formaldehyde condensation. Using these antibodies and a FuA-bovine serum albumine conjugate, a competitive indirect enzyme immunoassay (EIA) was established. The antiserum obtained from one rabbit enabled highly sensitive detection of FuA, with an IC50 level and detection limit of the standard curve of 3.3 ng/ml and approx. 1 ng/ml, respectively. The EIA was very specific for FuA, with 1.3% cross-reactivity with FuB. Several other lysergic acid derivatives (ergonovine, ergotamine, alpha-ergocryptine) were tested but did not cross-react in the FuA EIA. This is the first description of antibodies against FuA and the first development of an EIA for FuA.

摘要

多克隆抗体针对呋咱 A（FuA）从兔子获得免疫后，用呋咱关键孔石鳖血蓝蛋白结合物甲醛缩合。使用这些抗体和 FuA-牛血清白蛋白结合物，建立了竞争间接酶免疫测定法（EIA）。从一只兔子获得的抗血清能够高度敏感地检测 FuA，标准曲线的 IC50 水平和检测限分别为 3.3ng/ml 和约 1ng/ml。EIA 对 FuA 非常特异，与 FuB 的交叉反应率为 1.3%。其他几种麦角酸衍生物（麦角新碱、麦角胺、α-麦角隐亭）进行了测试，但在 FuA EIA 中没有交叉反应。这是针对 FuA 的抗体的首次描述，也是针对 FuA 的 EIA 的首次开发。