Abramson D
Agriculture and Agri-Food Canada, Cereal Research Center, Winnipeg, Canada.
J AOAC Int. 1996 Nov-Dec;79(6):1325-9.
Polyclonal antibodies against the mycotoxin citrinin were raised in rabbits after immunization with citrinin conjugated to keyhole limpet hemocyanin. The antibodies were used in a competitive indirect enzyme immunoassay (EIA) with citrinin coupled to glucose oxidase as the solid-phase antigen for coating microtiter plates. Detection limits of this indirect EIA for citrinin ranged from 0.4 to 0.8 ng/mL for buffer solutions. Recoveries of citrinin added to ground barley at 100-2000 ng/g ranged from 105 to 112%, with coefficients of variation between 4.5 and 12%. A direct competitive EIA also was established, with citrinin coupled to horseradish peroxidase as the labeled antigen. Detection limits of this direct EIA for citrinin ranged from 2 to 4 ng/mL for buffer solutions. Recoveries of citrinin added to ground barley at 500-2000 ng/g ranged from 108 to 111%, with coefficients of variation between 8.4 and 26.9%. In naturally contaminated barley samples assayed with the indirect EIA, optimum extraction of citrinin was obtained in 30 min, and only one extraction was necessary to recover 72-76% of the analyte.
用与钥孔血蓝蛋白偶联的桔霉素免疫家兔后，制备了抗真菌毒素桔霉素的多克隆抗体。这些抗体用于竞争性间接酶免疫分析（EIA），其中与葡萄糖氧化酶偶联的桔霉素作为固相抗原包被微量滴定板。该间接EIA对缓冲溶液中桔霉素的检测限为0.4至0.8 ng/mL。添加到粉碎大麦中浓度为100 - 2000 ng/g的桔霉素回收率为105%至112%，变异系数在4.5%至12%之间。还建立了直接竞争性EIA，其中与辣根过氧化物酶偶联的桔霉素作为标记抗原。该直接EIA对缓冲溶液中桔霉素的检测限为2至4 ng/mL。添加到粉碎大麦中浓度为500 - 2000 ng/g的桔霉素回收率为108%至111%，变异系数在8.4%至26.9%之间。在用间接EIA分析的天然污染大麦样品中，30分钟时桔霉素的提取效果最佳，只需一次提取就能回收72%至76%的分析物。
