真菌毒素产生菌的分子检测：最新综述。

Molecular detection of ochratoxin A producers: an updated review.

机构信息

Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350, Freising, Germany,

出版信息

Mycotoxin Res. 2006 Mar;22(1):48-53. doi: 10.1007/BF02954557.

DOI:10.1007/BF02954557

PMID:23605501

Abstract

Ochratoxin A (OTA) can be detected worldwide from various food and feed sources. It is produced byPenicillium nordicum andP. verrucosum as well as by variousAspergillus species, withA. ochraceus andA. carbonarius as the predominant producers. Various pairs of PCR primers based on AFLP, RAPD as well as primers specific to ribosomal RNA and genes coding for calmodulin and OTA biosynthetic pathway components were recently developed to detect and identify OTA producers in conventional and real-time PCR assays. Application of such assays in contaminated samples was demonstrated only in few cases. The current review gives an updated overview over the methods at hand.

摘要

赭曲霉毒素 A（OTA）可从各种食品和饲料来源中在全球范围内检测到。它是由青霉属的北方青霉和疣孢青霉以及各种曲霉属产生的，其中赭曲霉和炭黑曲霉是主要的产生菌。最近，已经开发了各种基于 AFLP、RAPD 的 PCR 引物对，以及针对核糖体 RNA 以及编码钙调蛋白和 OTA 生物合成途径成分的基因的引物对，用于在常规和实时 PCR 检测中检测和鉴定 OTA 产生菌。这些检测方法在污染样本中的应用仅在少数情况下得到了证实。目前的综述提供了对手头方法的最新概述。