从出芽短梗霉中分离、结构鉴定及三种新真菌毒素的毒理学特性研究。

Isolation, structural and toxicological characterization of three new mycotoxins produced by the fungusAureobasidium pullulans.

机构信息

Institut für physiologische Chemie, Universität Mainz, D-6500, Mainz.

出版信息

Mycotoxin Res. 1993 Mar;9(1):13-21. doi: 10.1007/BF03192227.

DOI:10.1007/BF03192227

Abstract

3 substances, B1, B2, and E1 were isolated from culture medium extracts ofAureobasidium pullulans by reversed phase liquid chromatography and subsequent liquid chromatographic purification steps on silica gel.The 3 compounds inhibited the metabolism ofSaccharomyces cerevisiae and showed toxic effects in the growth inhibition test toEscherichia coli andBacillus subtilis.Elementary analysis and mass spectroscopical methods revealed sum formulas of C23H22O6, C22H20O6 and C24H28O3 for B1 B2, and E1 and molecular weights of 394, 380, and 364, respectively. Mass spectroscopical, UV-, IR-,(13)C-NMR, and(1)H-NMR-spectroscopical investigations revealed polycyclic, non-aromatic compounds containing several carbonyl functions and double bonds and, most notably, spiroepoxy-functions, in the case of B1 and B2.

摘要

从出芽短梗霉培养液提取物中，经反相液相色谱和硅胶随后的液相色谱纯化步骤，分离到 3 种物质 B1、B2 和 E1。这 3 种化合物抑制酿酒酵母的代谢，在大肠杆菌和枯草芽孢杆菌的生长抑制试验中显示出毒性作用。元素分析和质谱方法揭示了 B1、B2 和 E1 的分子式分别为 C23H22O6、C22H20O6 和 C24H28O3，分子量分别为 394、380 和 364。质谱、紫外、红外、（13）C-NMR 和（1）H-NMR 光谱研究揭示了 B1 和 B2 为含有多个羰基和双键的多环非芳香化合物，特别是具有螺环氧官能的化合物。